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通过与 Triton X-114 进行相分配鉴定梅毒螺旋体苍白亚种整合膜蛋白的病原体特异性。

Pathogen specificity of Treponema pallidum subsp. pallidum integral membrane proteins identified by phase partitioning with Triton X-114.

作者信息

Radolf J D, Norgard M V

机构信息

Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.

出版信息

Infect Immun. 1988 Jul;56(7):1825-8. doi: 10.1128/iai.56.7.1825-1828.1988.

DOI:10.1128/iai.56.7.1825-1828.1988
PMID:3290110
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259484/
Abstract

The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (detergent-phase proteins) appeared to be antigenically related to proteins of the nonpathogens. Protease-resistant material similar to lipopolysaccharide was identified in the detergent phase from T. phagedenis biotype Reiter but was not detected in T. pallidum.

摘要

利用非离子去污剂Triton X-114进行相分配和免疫印迹分析，对梅毒螺旋体苍白亚种及四种可培养的非致病性密螺旋体的抗原保守蛋白进行了研究。通过相分配鉴定出的梅毒螺旋体整合膜蛋白（去污剂相蛋白）中，没有一种似乎与非病原体的蛋白存在抗原相关性。在蚀疮艾肯菌生物型赖特氏菌的去污剂相中鉴定出了类似于脂多糖的蛋白酶抗性物质，但在梅毒螺旋体中未检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/6611c80e4fdd/iai00079-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/21e0d6f34d56/iai00079-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/da89c7017685/iai00079-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/6f74684ec150/iai00079-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/6611c80e4fdd/iai00079-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/21e0d6f34d56/iai00079-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/da89c7017685/iai00079-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/6f74684ec150/iai00079-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfed/259484/6611c80e4fdd/iai00079-0163-a.jpg

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Pathogen specificity of Treponema pallidum subsp. pallidum integral membrane proteins identified by phase partitioning with Triton X-114.通过与 Triton X-114 进行相分配鉴定梅毒螺旋体苍白亚种整合膜蛋白的病原体特异性。
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