Barbosa Mariane C F, Lima Daniela C, Reis Caio L B, Reis Ana L M, Rigo Darlan, Segato Raquel A B, Storrer Carmen L M, Küchler Erika C, de Oliveira Daniela S B
Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Minas Gerais, Brazil.
School of Health Sciences, Positivo University, Curitiba, Brazil.
Int J Paediatr Dent. 2020 Sep;30(5):642-649. doi: 10.1111/ipd.12631. Epub 2020 Feb 28.
BACKGROUND & AIM: To investigate the association between the genetic polymorphisms FokI (rs2228570) and BglI (rs739837) in vitamin D receptor (VDR) with dental caries and gingivitis susceptibility.
This study included 353 Brazilian children (8 to 11 years old). Dental caries was assessed using ICDAS (International System for Detection and Assessment of Carious Lesions) and gingival bleeding using Community Periodontal Index (CPI). The presence of visible biofilm was also evaluated. DNA was extracted from saliva, and real-time PCR was used to evaluate genetic polymorphisms in VDR: rs2228570 (FokI, A>G/Met>Thr) and rs739837 (BglI, G>T). Dental caries was evaluated as a continuous data (mean and standard deviation-SD) and was also categorized (ICDAS versus ICDAS or ICDAS versus ICDAS). Gingivitis was categorized in with and without. One-way ANOVA was used for comparisons of caries among genotypes. Chi-square test, logistic regression, and haplotype analysis were performed (P < .05).
Biofilm was associated with dental caries susceptibility and gingivitis (P < .05). The mean distribution of the caries lesions and cavitated caries lesions among FokI and BgII genotypes were not statistically significant (P > .05). Genotype distributions among caries groups (in the two different cut-offs) and among gingivitis and non-gingivitis groups were not statistically significant (P > .05).
The polymorphisms FokI and BglI in VDR were not associated with dental caries or gingivitis.
研究维生素D受体（VDR）基因多态性FokI（rs2228570）和BglI（rs739837）与龋齿及牙龈炎易感性之间的关联。
本研究纳入353名巴西儿童（8至11岁）。采用国际龋病检测与评估系统（ICDAS）评估龋齿情况，使用社区牙周指数（CPI）评估牙龈出血情况。同时评估可见生物膜的存在情况。从唾液中提取DNA，采用实时聚合酶链反应评估VDR基因多态性：rs2228570（FokI，A>G/甲硫氨酸>苏氨酸）和rs739837（BglI，G>T）。将龋齿情况评估为连续数据（均值和标准差-SD），并进行分类（ICDAS对ICDAS或ICDAS对ICDAS）。将牙龈炎分为有和无两类。采用单因素方差分析比较各基因型间的龋齿情况。进行卡方检验、逻辑回归和单倍型分析（P<0.05）。
生物膜与龋齿易感性和牙龈炎相关（P<0.05）。FokI和BgII基因型之间龋齿病变和龋洞性龋齿病变的平均分布无统计学意义（P>0.05）。龋齿组（两种不同分类标准）、牙龈炎组和非牙龈炎组之间的基因型分布无统计学意义（P>0.05）。
VDR基因的FokI和BglI多态性与龋齿或牙龈炎无关。
