Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China.
Environ Sci Technol. 2020 Oct 6;54(19):12366-12375. doi: 10.1021/acs.est.0c03691. Epub 2020 Sep 20.
High-resolution mass spectrometry (HRMS) analysis with the assistance of molecular networking was used to investigate intracellular toxin profiles of five () strains sampled from the north Yellow Sea and South China Sea. Mice were used as a model species for testing the acute toxicity of intracellular okadaic acid (OA) and dinophysistoxins (DTXs) in free and esterified states. Results showed that OA and DTX1 esterified derivatives were detected in all samples, accounting for 55%-96% of total toxins in five strains. A total of 24 esters and 1 stereoisomer of DTX1 (35S DTX1) were identified based on molecular networking and MS data analysis, 15 esters of which have been reported first. All strains displayed specific toxin profiles, and preliminary analysis suggested that toxin profiles of the five strains might be region-related. Moreover, acute toxicity in mice suggested higher toxicity of esters compared with free toxins, which highlights the importance and urgency of attention to esterified toxins in .
采用高分辨质谱（HRMS）分析，并结合分子网络技术，研究了来自黄海北部和南海的 5 株（）菌株的细胞内毒素谱。以小鼠为模型物种，检测了细胞内 okadaic 酸（OA）和戴奥辛毒素（DTXs）在游离态和酯化态下的急性毒性。结果表明，所有 菌株均检测到 OA 和 DTX1 酯化衍生物，占 5 株菌总毒素的 55%-96%。基于分子网络和 MS 数据分析，共鉴定出 24 个 DTX1 酯和 1 个 DTX1 立体异构体（35S DTX1），其中 15 个酯为首次报道。所有 菌株均显示出特定的毒素谱，初步分析表明，这 5 株 菌株的毒素谱可能与地区有关。此外，小鼠的急性毒性表明，酯化毒素比游离毒素的毒性更高，这凸显了关注 中酯化毒素的重要性和紧迫性。
