Dubey Sushil, Yadav Chandrabhan, Bajpeyee Anand, Singh Mohan P
Centre of Biotechnology, University of Allahabad, Prayagraj, Uttar Pradesh, India.
Diabetes Metab Syndr Obes. 2020 Aug 24;13:3035-3046. doi: 10.2147/DMSO.S265798. eCollection 2020.
The antidiabetic effects of the on liver and kidney were unexplored. The present study assessed the vital effects of aqueous extract (PFA-extract) on liver and kidney function in diabetic rats model.
The albino Wistar rats were divided into five groups with six animals in each. Group, I, II, and III were normal, diabetic, and drug control, respectively, and groups IV and V were test groups. As treatment dose, group II received saline, and group III (drug control) received metformin in 100 mg/kg of body weight as a standard drug. Whereas, group IV (D1) and V (D2) were test groups, received PFA-extract in 250 mg/kg, and 500 mg/kg of body weight, respectively. The changes in body weight, blood glucose level (BGL), lipid profile, liver, and kidney biochemical parameters were evaluated.
The PFA-extract dose at 500 mg/kg in D2 groups showed very good effects. The body weight was recovered by 97.9% and blood glucose level (BGL) was reduced to 53% in the D2 group. For the lipid profile, total cholesterol (TC), triglyceride (TG), and high-density lipoprotein (HDL) were estimated in blood plasma and their values were 92.31±3.788, 80.85±8.962, and 31.35±1.781, respectively. PFA-extract improved the liver function for which aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were evaluated and recorded to their normal range in D2 group which were 132.01±5.553, 58.63±4.390, and 143.26±2.423, respectively. For the kidney function test, creatinine (CRT), blood urea nitrogen (BUN), and uric acid were evaluated and in the D2 group, it was significantly reduced ie, 0.656±0.0707, 15.13±1.463, and 6.27±0.325, respectively.
These attributes of PFA-extract make it a potential natural agent to provide protection to the liver and kidney and also reduce the BGL and control the total lipid in type 1 diabetes condition.
[提取物名称]对肝脏和肾脏的抗糖尿病作用尚未得到研究。本研究评估了[提取物名称]水提取物（PFA提取物）对糖尿病大鼠模型肝脏和肾脏功能的重要影响。
将白化Wistar大鼠分为五组，每组六只动物。第一组、第二组和第三组分别为正常组、糖尿病组和药物对照组，第四组和第五组为试验组。作为治疗剂量，第二组接受生理盐水，第三组（药物对照组）接受100mg/kg体重的二甲双胍作为标准药物。而第四组（D1）和第五组（D2）为试验组，分别接受250mg/kg和500mg/kg体重的PFA提取物。评估体重、血糖水平（BGL）、血脂、肝脏和肾脏生化参数的变化。
D2组中500mg/kg的PFA提取物剂量显示出非常好的效果。D2组体重恢复了97.9%，血糖水平（BGL）降低到了53%。对于血脂，测定了血浆中的总胆固醇（TC）、甘油三酯（TG）和高密度脂蛋白（HDL），其值分别为92.31±3.788、80.85±8.962和31.35±1.781。PFA提取物改善了肝功能，为此评估了天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP），并在D2组中记录到其正常范围，分别为132.01±5.553、58.63±4.390和143.26±2.423。对于肾功能测试，评估了肌酐（CRT）、血尿素氮（BUN）和尿酸，在D2组中，它们显著降低，分别为0.656±0.0707、15.13±1.463和6.27±0.325。
PFA提取物的这些特性使其成为一种潜在的天然药物，可保护肝脏和肾脏，还可降低1型糖尿病患者的BGL并控制总血脂。
