Kanukurti Jyothirmayi, Mohammed Noorjahan, Sreedevi N N, Khan Siraj Ahmed, Baba K S S Sai, Bhaskar M Vijaya, Satish O Sai, Naushad Shaik Mohammad, Mohan Iyyapu Krishna
Department of Biochemistry, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Department of Cardiology, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
J Lab Physicians. 2020 Aug;12(2):126-132. doi: 10.1055/s-0040-1716608. Epub 2020 Sep 1.
Heart failure is a complex cardiovascular disease with a variety of etiologies and heterogeneity. The N-terminal pro-B-type natriuretic peptide (NT-proBNP) value has limited usefulness in diagnosing heart failure with preserved ejection fraction (HFpEF). The aim of the present study is to evaluate serum Galectin-3 as a diagnostic biomarker in patients with HFpEF and to compare Galectin-3 with NT-proBNP levels. A cross-sectional case-control study including 63 cases of heart failure with ejection fraction ≥50% confirmed by echocardiography. NT-proBNP levels in serum were measured by electrochemiluminescence immunoassay and Galectin-3 levels in serum were measured by using an enzyme-linked-immunosorbent serologic assay kit. The median levels of serum Galectin-3 and NT-proBNP in patients were significantly higher than those of controls (26.59 vs. 5.27 and 927 vs. 49.3, < 0.0001). A positive correlation was observed between serum levels of Galection-3 and NT-ProBNP ( : 0.21, = 0.048). At cut-off values of 10.1 ng/mL and 160 pg/mL, serum Galectin-3 has 77.78% sensitivity, 95% specificity with an area under the curve (AUC) of 0.93, and serum NT-proBNP has 71.43% sensitivity, 100% specificity with an AUC of 0.87, respectively, for diagnosing HFpEF. The comparison of receiver operating characteristics curves showed that Galectin-3 has better AUC compared with NT-proBNP in diagnosing HFpEF. Serum Galectin-3 showed a positive correlation with NT-proBNP and lipid parameters. Galectin-3 with higher sensitivity and AUC can be used as a valuable biomarker for the diagnosis of HFpEF. Simultaneous testing of both Galectin-3 and NT-proBNP can further improve the detection of patients with HFpEF.
心力衰竭是一种病因多样且具有异质性的复杂心血管疾病。N末端B型利钠肽原（NT-proBNP）值在诊断射血分数保留的心力衰竭（HFpEF）方面的作用有限。本研究旨在评估血清半乳糖凝集素-3作为HFpEF患者诊断生物标志物的价值，并将半乳糖凝集素-3与NT-proBNP水平进行比较。一项横断面病例对照研究，纳入了63例经超声心动图证实射血分数≥50%的心力衰竭患者。采用电化学发光免疫分析法测定血清NT-proBNP水平，使用酶联免疫吸附血清学检测试剂盒测定血清半乳糖凝集素-3水平。患者血清半乳糖凝集素-3和NT-proBNP的中位数水平显著高于对照组（26.59对5.27以及927对49.3，<0.0001）。观察到半乳糖凝集素-3和NT-proBNP的血清水平之间存在正相关（r：0.21，P = 0.048）。在截断值分别为10.1 ng/mL和160 pg/mL时，血清半乳糖凝集素-3诊断HFpEF的灵敏度为77.78%，特异性为95%，曲线下面积（AUC）为0.93，血清NT-proBNP的灵敏度为71.43%，特异性为100%，AUC为0.87。受试者工作特征曲线的比较表明，在诊断HFpEF方面，半乳糖凝集素-3的AUC比NT-proBNP更好。血清半乳糖凝集素-3与NT-proBNP和脂质参数呈正相关。半乳糖凝集素-3具有较高的灵敏度和AUC，可作为诊断HFpEF的有价值生物标志物。同时检测半乳糖凝集素-3和NT-proBNP可进一步提高HFpEF患者的检出率。
