Wang Jian, Hua Xuanwen, Guo Changliang, Liu Wenhao, Jia Shu
School of Physics, Harbin Institute of Technology, Harbin, Heilongjiang, China.
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Optica. 2020 Jul 20;7(7):790-793. doi: 10.1364/optica.389894.
We introduce Airy-beam tomographic microscopy (ATM) for high-resolution, volumetric, inertia-free imaging of biological specimens. The work exploits the highly adjustable Airy trajectories in the three-dimensional (3D) space, transforming the conventional telecentric wide-field imaging scheme that requires sample or focal-plane scanning to acquire 3D information. The results present a consistent near-diffraction-limited 3D resolution across a tenfold extended imaging depth compared to wide-field microscopy. We anticipate the strategy to not only offer a promising paradigm for 3D optical microscopy, but also be translated to other non-optical waveforms.
我们介绍了用于生物样本高分辨率、体积成像且无惯性的艾里光束断层扫描显微镜（ATM）。这项工作利用了三维（3D）空间中高度可调节的艾里轨迹，改变了传统的远心宽场成像方案，该方案需要样本或焦平面扫描来获取3D信息。与宽场显微镜相比，结果显示在十倍扩展的成像深度上具有一致的近衍射极限3D分辨率。我们预计该策略不仅为3D光学显微镜提供了一个有前景的范例，还能转化到其他非光学波形上。
