State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China.
Crit Rev Biotechnol. 2020 Dec;40(8):1232-1249. doi: 10.1080/07388551.2020.1809990. Epub 2020 Sep 9.
In recent years, there has been increasing interest in microbial biotechnology for the production of value-added compounds from renewable resources. species have been proposed as a suitable workhorse for high-value secondary metabolite production because of their unique characteristics for fast growth on sustainable carbon sources, a clear inherited background, versatile intrinsic metabolism with diverse enzymatic capacities, and their robustness in an extreme environment. It has also been demonstrated that metabolically engineered strains can produce several industrially valuable aromatic chemicals and natural products such as phenazines, polyhydroxyalkanoates, rhamnolipids, and insecticidal proteins from renewable feedstocks with remarkably high yields suitable for commercial application. In this review, we summarize cell factory construction in for the biosynthesis of native and non-native bioactive compounds in . , . , . , as well as pharmaceutical proteins production by . . Additionally, some novel strategies together with metabolic engineering strategies in order to improve the biosynthetic abilities of as an ideal chassis are discussed. Finally, we proposed emerging opportunities, challenges, and essential strategies to enable the successful development of as versatile microbial cell factories for the bioproduction of diverse bioactive compounds.
近年来，人们对利用微生物生物技术从可再生资源中生产增值化合物越来越感兴趣。由于其在可持续碳源上快速生长、清晰的遗传背景、多功能内在代谢和多样化的酶能力以及在极端环境中的稳健性等独特特性，已被提议作为生产高价值次级代谢产物的合适工程菌。已经证明，经过代谢工程改造的 菌株可以从可再生原料中生产出几种具有工业价值的芳香族化学品和天然产物，如吩嗪、聚羟基烷酸酯、鼠李糖脂和杀虫蛋白，其产量非常高，适合商业应用。在这篇综述中，我们总结了在 中构建细胞工厂以合成天然和非天然生物活性化合物的情况，包括 、 、 、 、 等，以及利用 生产药用蛋白。此外，还讨论了一些新的策略以及代谢工程策略，以提高 作为理想底盘的生物合成能力。最后，我们提出了新兴的机会、挑战和必要的策略，以使 成功发展为用于生产多种生物活性化合物的多功能微生物细胞工厂。
