Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.
Appl Microbiol Biotechnol. 2018 Oct;102(20):8685-8705. doi: 10.1007/s00253-018-9289-6. Epub 2018 Aug 14.
Recent progress in synthetic and systems metabolic engineering technologies has explored the potential of microbial cell factories for the production of industrially relevant bulk and fine chemicals from renewable biomass resources in an eco-friendly manner. Corynebacterium glutamicum, a workhorse for industrial amino acid production, has currently evolved into a promising microbial platform for bioproduction of various natural and non-natural chemicals from renewable feedstocks. Notably, it has been recently demonstrated that metabolically engineered C. glutamicum can overproduce several commercially valuable aromatic and related chemicals such as shikimate, 4-hydroxybenzoate, and 4-aminobenzoate from sugars at remarkably high titer suitable to commercial application. On the other hand, overexpression and/or extension of its endogenous metabolic pathways by integrating heterologous metabolic pathways enabled production of structurally intricate and valuable natural chemicals like plant polyphenols, carotenoids, and fatty acids. In this review, we summarize recent advances in metabolic engineering of C. glutamicum for production of those value-added aromatics and other natural products, which highlights high potential and the versatility of this microbe for bioproduction of diverse chemicals.
近年来，合成和系统代谢工程技术的进步探索了微生物细胞工厂在环保的方式下，利用可再生生物质资源生产工业相关的大宗化学品和精细化学品的潜力。谷氨酸棒杆菌作为工业氨基酸生产的主力，目前已发展成为一种很有前途的微生物平台，可以从可再生原料生产各种天然和非天然化学品。值得注意的是，最近的研究表明，经过代谢工程改造的谷氨酸棒杆菌可以从糖中高浓度、适合商业应用的水平过量生产几种具有商业价值的芳香族和相关化学品，如莽草酸、4-羟基苯甲酸和 4-氨基苯甲酸。另一方面，通过整合异源代谢途径，过表达和/或扩展其内源代谢途径，使得能够生产结构复杂且有价值的天然化学品，如植物多酚、类胡萝卜素和脂肪酸。在本文中，我们总结了谷氨酸棒杆菌在生产这些附加值芳香族化合物和其他天然产物方面的代谢工程的最新进展，这突显了该微生物在生产各种化学品方面的高潜力和多功能性。
