Weimer Anna, Kohlstedt Michael, Volke Daniel C, Nikel Pablo I, Wittmann Christoph
Institute of Systems Biotechnology, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.
Appl Microbiol Biotechnol. 2020 Sep;104(18):7745-7766. doi: 10.1007/s00253-020-10811-9. Epub 2020 Aug 13.
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism, adapted to withstand physicochemical stress, and the capacity to thrive in harsh environments. Owing to these characteristics, there is a growing interest in this microbe for industrial use, and the corresponding research has made rapid progress in recent years. Hereby, strong drivers are the exploitation of cheap renewable feedstocks and waste streams to produce value-added chemicals and the steady progress in genetic strain engineering and systems biology understanding of this bacterium. Here, we summarize the recent advances and prospects in genetic engineering, systems and synthetic biology, and applications of P. putida as a cell factory. KEY POINTS: • Pseudomonas putida advances to a global industrial cell factory. • Novel tools enable system-wide understanding and streamlined genomic engineering. • Applications of P. putida range from bioeconomy chemicals to biosynthetic drugs.
恶臭假单胞菌是一种革兰氏阴性、杆状细菌，可在多种生态栖息地中发现。其广泛存在归因于其极其多样的代谢，能够适应物理化学压力，以及在恶劣环境中茁壮成长的能力。由于这些特性，人们对这种微生物在工业上的应用兴趣日益浓厚，近年来相关研究取得了快速进展。在此，主要驱动力是利用廉价的可再生原料和废物流来生产增值化学品，以及在该细菌的基因工程菌株和系统生物学理解方面的稳步进展。在此，我们总结了恶臭假单胞菌作为细胞工厂在基因工程、系统与合成生物学以及应用方面的最新进展和前景。要点：• 恶臭假单胞菌正向全球工业细胞工厂迈进。• 新型工具实现了全系统理解和简化的基因组工程。• 恶臭假单胞菌的应用范围从生物经济化学品到生物合成药物。
