• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Circular DNA of 3T6R50 double minute chromosomes.

作者信息

van der Bliek A M, Lincke C R, Borst P

机构信息

Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.

出版信息

Nucleic Acids Res. 1988 Jun 10;16(11):4841-51. doi: 10.1093/nar/16.11.4841.

DOI:10.1093/nar/16.11.4841
PMID:3290843
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336700/
Abstract

In pulsed field gradient gel electrophoresis (PFGE) the intact deproteinized circular DNA of Mycoplasma (800 kb) and Escherichia coli (4700 kb) remains trapped in the slot. We show here that gamma-irradiation of the DNA in agarose plugs is a convenient method to partially convert these circles into full-length linears, migrating with the expected mobility in PFGE. We have used this method to study the structure of Double Minute chromosomes (DMs) from the methotrexate (MTX)-resistant mouse cell line 3T6R50. Intact deproteinized DM DNA is immobile in these gels, but is converted into a single band of about 2500 kb by either gamma-irradiation, DNaseI in the presence of Mn2+, or restriction enzymes. We conclude that the DM DNA in 3T6R50 cells consists of a homogeneous population of 2500-kb circles.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/49077da01d62/nar00154-0107-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/14a91ec09360/nar00154-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/cf7cc9c72bf7/nar00154-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/1b94886dc5af/nar00154-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/32abd52a55fd/nar00154-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/49077da01d62/nar00154-0107-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/14a91ec09360/nar00154-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/cf7cc9c72bf7/nar00154-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/1b94886dc5af/nar00154-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/32abd52a55fd/nar00154-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ec1/336700/49077da01d62/nar00154-0107-b.jpg

相似文献

1
Circular DNA of 3T6R50 double minute chromosomes.
Nucleic Acids Res. 1988 Jun 10;16(11):4841-51. doi: 10.1093/nar/16.11.4841.
2
DNA content and structure of (double) minutes of a methotrexate-resistant cell line.
Histochemistry. 1989;93(1):87-92. doi: 10.1007/BF00266852.
3
Structure of amplified DNA, analyzed by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分析的扩增DNA结构。
J Cell Biochem. 1987 Aug;34(4):247-58. doi: 10.1002/jcb.240340404.
4
Double minute chromosomes carrying the human multidrug resistance 1 and 2 genes are generated from the dimerization of submicroscopic circular DNAs in colchicine-selected KB carcinoma cells.携带人类多药耐药1和2基因的双微小染色体是由秋水仙碱选择的KB癌细胞中亚显微环状DNA二聚化产生的。
Mol Biol Cell. 1992 May;3(5):507-20. doi: 10.1091/mbc.3.5.507.
5
Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis.脉冲场梯度电泳中大型环状DNA“异常”迁移率的表征
Nucleic Acids Res. 1988 Feb 11;16(3):925-39. doi: 10.1093/nar/16.3.925.
6
Single-molecule measurements of trapped and migrating circular DNA during electrophoresis in agarose gels.琼脂糖凝胶电泳过程中捕获和迁移的环状DNA的单分子测量。
Electrophoresis. 2006 Nov;27(22):4396-407. doi: 10.1002/elps.200600347.
7
Chromosome breakpoints near CpG islands in double minutes.
Gene. 1998 Sep 18;218(1-2):121-8. doi: 10.1016/s0378-1119(98)00398-9.
8
Estimation of circular DNA size using gamma-irradiation and pulsed-field gel electrophoresis.
Anal Biochem. 1989 Feb 15;177(1):110-4. doi: 10.1016/0003-2697(89)90023-7.
9
Separation of large circular DNA by electrophoresis in agarose gels.通过琼脂糖凝胶电泳分离大型环状DNA。
Biotechnol Prog. 2002 Jan-Feb;18(1):82-7. doi: 10.1021/bp010135o.
10
Pulsed-field gel electrophoresis of circular DNA.环状DNA的脉冲场凝胶电泳
Nucleic Acids Res. 1989 Jun 12;17(11):4359-65. doi: 10.1093/nar/17.11.4359.

引用本文的文献

1
Profiling oncogenic extra-chromosomal DNA in cancer.
Nat Genet. 2022 Nov;54(11):1591-1592. doi: 10.1038/s41588-022-01193-x.
2
Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH.通过 CRISPR-CATCH 对人类染色体外 DNA 进行靶向分析。
Nat Genet. 2022 Nov;54(11):1746-1754. doi: 10.1038/s41588-022-01190-0. Epub 2022 Oct 17.
3
Gene regulation on extrachromosomal DNA.染色体外 DNA 的基因调控。
Nat Struct Mol Biol. 2022 Aug;29(8):736-744. doi: 10.1038/s41594-022-00806-7. Epub 2022 Aug 10.

本文引用的文献

1
Isolation and expression of an altered mouse dihydrofolate reductase cDNA.一种改变的小鼠二氢叶酸还原酶cDNA的分离与表达
Proc Natl Acad Sci U S A. 1983 May;80(9):2495-9. doi: 10.1073/pnas.80.9.2495.
2
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
Nucleic Acids Res. 1984 Jul 25;12(14):5647-64. doi: 10.1093/nar/12.14.5647.
3
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
4
ecDNA hubs drive cooperative intermolecular oncogene expression.染色体外环状DNA中心驱动分子间癌基因的协同表达。
Nature. 2021 Dec;600(7890):731-736. doi: 10.1038/s41586-021-04116-8. Epub 2021 Nov 24.
5
Extrachromosomal circular DNA-based amplification and transmission of herbicide resistance in crop weed .作物杂草中外源环状 DNA 的扩增和除草剂抗性的传播。
Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3332-3337. doi: 10.1073/pnas.1719354115. Epub 2018 Mar 12.
6
Physical map and genome sequencing survey of Mycoplasma haemofelis (Haemobartonella felis).溶血支原体（血巴尔通体属猫血巴尔通体）的物理图谱及基因组测序调查
Infect Immun. 2003 Jun;71(6):3657-62. doi: 10.1128/IAI.71.6.3657-3662.2003.
7
Different roles for nonhomologous end joining and homologous recombination following replication arrest in mammalian cells.哺乳动物细胞复制停滞后非同源末端连接和同源重组的不同作用。
Mol Cell Biol. 2002 Aug;22(16):5869-78. doi: 10.1128/MCB.22.16.5869-5878.2002.
8
Frequent amplification of a short chain dehydrogenase gene as part of circular and linear amplicons in methotrexate resistant Leishmania.
Nucleic Acids Res. 1993 Sep 11;21(18):4305-12. doi: 10.1093/nar/21.18.4305.
9
Multiple replicons constituting the genome of Pseudomonas cepacia 17616.构成洋葱伯克霍尔德菌17616基因组的多个复制子。
J Bacteriol. 1994 Jul;176(13):4034-42. doi: 10.1128/jb.176.13.4034-4042.1994.
10
DNA content and structure of (double) minutes of a methotrexate-resistant cell line.
Histochemistry. 1989;93(1):87-92. doi: 10.1007/BF00266852.
Cell. 1984 May;37(1):67-75. doi: 10.1016/0092-8674(84)90301-5.
4
Relationship of amplified dihydrofolate reductase genes to double minute chromosomes in unstably resistant mouse fibroblast cell lines.不稳定抗性小鼠成纤维细胞系中扩增的二氢叶酸还原酶基因与双微体染色体的关系。
Mol Cell Biol. 1981 Dec;1(12):1077-83. doi: 10.1128/mcb.1.12.1077-1083.1981.
5
Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.利用限制性内切酶和电子显微镜对猪鼻支原体基因组进行分析。
J Bacteriol. 1982 May;150(2):788-94. doi: 10.1128/jb.150.2.788-794.1982.
6
The genes coding for the cytoskeletal proteins actin and vimentin in warm-blooded vertebrates.温血脊椎动物中编码细胞骨架蛋白肌动蛋白和波形蛋白的基因。
EMBO J. 1982;1(2):167-71. doi: 10.1002/j.1460-2075.1982.tb01142.x.
7
Size and structure of the Mycoplasma hominis H39 chromosome.人型支原体H39染色体的大小与结构。
J Mol Biol. 1967 Jan 28;23(2):191-9. doi: 10.1016/s0022-2836(67)80026-3.
8
Deoxyribonucleic acid nucleases. II. The effects of metals on the mechanism of action of deoxyribonuclease I.脱氧核糖核酸酶。II. 金属对脱氧核糖核酸酶I作用机制的影响。
J Biol Chem. 1968 Sep 10;243(17):4409-16.
9
The effects of gamma-radiation in DNA.γ辐射对DNA的影响。
Curr Top Radiat Res Q. 1973 Dec;9(2):165-245.
10
Ultrastructural features of minute chromosomes in a methotrexate-resistant mouse 3T3 cell line.甲氨蝶呤耐药小鼠3T3细胞系微小染色体的超微结构特征
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1126-30. doi: 10.1073/pnas.82.4.1126.