调节蛋白对DNA结合位点的选择：LexA蛋白和精氨酸阻遏蛋白采用不同策略实现功能特异性。
Selection of DNA binding sites by regulatory proteins: the LexA protein and the arginine repressor use different strategies for functional specificity.
作者信息
Berg O G
机构信息
Department of Molecular Biology, Uppsala University Biomedical Center, Sweden.
出版信息
Nucleic Acids Res. 1988 Jun 10;16(11):5089-105. doi: 10.1093/nar/16.11.5089.
Abstract
The DNA sequences in the operator sites of the arginine regulon and of the SOS regulon have been subject to a statistical analysis. A quantitative correlation is found between the statistics of sequence choice and the activity at individual operator sites in both systems, as expected from theoretical considerations [Berg & von Hippel, J.Mol.Biol. (1987) 193, 723-750]. Based on these correlations it is possible to predict the effect of various sequence mutations. There is a significant difference in the slopes of the correlation lines between sequence and activity for the two systems. From this difference it can be expected that individual point mutations in the ARG boxes will have a much smaller effect on activity than similar changes in the SOS boxes. This difference may be related to a strong cooperative activity at tandem ARG boxes while the binding at SOS boxes appears to be mostly noncooperative.
摘要
已对精氨酸调节子和SOS调节子操纵基因位点中的DNA序列进行了统计分析。正如理论考虑所预期的那样[伯格和冯·希佩尔，《分子生物学杂志》（1987年）193卷，723 - 750页]，在这两个系统中，序列选择的统计数据与各个操纵基因位点的活性之间发现了定量相关性。基于这些相关性，可以预测各种序列突变的影响。两个系统中序列与活性之间的相关线斜率存在显著差异。由此可以预期，ARG框中的单个点突变对活性的影响将比SOS框中的类似变化小得多。这种差异可能与串联ARG框处的强协同活性有关，而SOS框处的结合似乎大多是非协同的。
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