Interventional Regenerative Medicine and Imaging Laboratory, Stanford University School of Medicine, Department of Radiology, Palo Alto, California 94304, United States.
Biionix (Bionic Materials, Implants & Interfaces) Cluster, Department of Internal Medicine, College of Medicine, University of Central Florida, Orlando, Florida 32827, United States.
Nano Lett. 2020 Oct 14;20(10):7220-7229. doi: 10.1021/acs.nanolett.0c02576. Epub 2020 Sep 21.
In the present study, we created a nanoscale platform that can deliver nutrients to pancreatic islets in a controlled manner. Our platform consists of a mesoporous silica nanoparticle (MSNP), which can be loaded with glutamine (G: an essential amino acid required for islet survival and function). To control the release of G, MSNPs were coated with a polydopamine (PD) layer. With the optimal parameters (0.5 mg/mL and 0.5 h), MSNPs were coated with a layer of PD, which resulted in a delay of G release from MSNPs over 14 d (57.4 ± 4.7% release). Following syngeneic renal subcapsule islet transplantation in diabetic mice, PDG-MSNPs improved the engraftment of islets (i.e., enhanced revascularization and reduced inflammation) as well as their function, resulting in re-establishment of glycemic control. Collectively, our data show that PDG-MSNPs can support transplanted islets by providing them with a controlled and sustained supply of nutrients.
在本研究中，我们创建了一个纳米级平台，能够以可控的方式将营养物质递送到胰岛。我们的平台由介孔硅纳米颗粒（MSNP）组成，它可以负载谷氨酰胺（G：胰岛存活和功能所必需的必需氨基酸）。为了控制 G 的释放，MSNPs 被包裹上聚多巴胺（PD）层。在最佳参数（0.5mg/mL 和 0.5h）下，MSNPs 被包裹上 PD 层，导致 G 从 MSNPs 中的释放延迟超过 14 天（57.4±4.7%的释放）。在糖尿病小鼠的同基因肾被膜下胰岛移植后，PDG-MSNPs 改善了胰岛的移植效果（即，增强了再血管化和减少了炎症）及其功能，从而重新建立了血糖控制。总的来说，我们的数据表明，PDG-MSNPs 可以通过为移植的胰岛提供受控和持续的营养物质供应来支持它们。
