Suppr超能文献

莱茵衣藻CC-1690的高度连续纳米孔基因组组装

Highly Contiguous Nanopore Genome Assembly of Chlamydomonas reinhardtii CC-1690.

作者信息

O'Donnell Samuel, Chaux Frederic, Fischer Gilles

机构信息

Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France

Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France.

出版信息

Microbiol Resour Announc. 2020 Sep 10;9(37):e00726-20. doi: 10.1128/MRA.00726-20.

Abstract

The current reference genome remains fragmented due to gaps stemming from large repetitive regions. To overcome the vast majority of these gaps, publicly available Oxford Nanopore Technology data were used to create a new reference-quality genome assembly containing only 21 contigs, 30/34 telomeric ends, and a genome size of 111 Mb.

摘要

由于来自大型重复区域的缺口,当前的参考基因组仍然是碎片化的。为了克服绝大多数这些缺口,利用公开可用的牛津纳米孔技术数据创建了一个新的参考质量基因组组装体,该组装体仅包含21个重叠群、34个端粒末端中的30个,基因组大小为111 Mb。

相似文献

1
Highly Contiguous Nanopore Genome Assembly of Chlamydomonas reinhardtii CC-1690.
Microbiol Resour Announc. 2020 Sep 10;9(37):e00726-20. doi: 10.1128/MRA.00726-20.
2
A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis.
Plant Commun. 2023 Mar 13;4(2):100493. doi: 10.1016/j.xplc.2022.100493. Epub 2022 Nov 17.
3
whole-genome assembly of a wild type yeast isolate using nanopore sequencing.
F1000Res. 2017 May 3;6:618. doi: 10.12688/f1000research.11146.2. eCollection 2017.
4
Highly Contiguous Genome Assemblies of 15 Species Generated Using Nanopore Sequencing.
G3 (Bethesda). 2018 Oct 3;8(10):3131-3141. doi: 10.1534/g3.118.200160.
5
Genome assembly using Nanopore-guided long and error-free DNA reads.
BMC Genomics. 2015 Apr 20;16(1):327. doi: 10.1186/s12864-015-1519-z.
6
Benchmarking of de novo assembly algorithms for Nanopore data reveals optimal performance of OLC approaches.
BMC Genomics. 2016 Aug 22;17 Suppl 7(Suppl 7):507. doi: 10.1186/s12864-016-2895-8.
8
EST assembly supported by a draft genome sequence: an analysis of the Chlamydomonas reinhardtii transcriptome.
Nucleic Acids Res. 2007;35(6):2074-83. doi: 10.1093/nar/gkm081. Epub 2007 Mar 13.
10
Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome.
Genome Res. 2015 Nov;25(11):1750-6. doi: 10.1101/gr.191395.115. Epub 2015 Oct 7.

引用本文的文献

2
Extraction and selection of high-molecular-weight DNA for long-read sequencing from Chlamydomonas reinhardtii.
PLoS One. 2024 Feb 8;19(2):e0297014. doi: 10.1371/journal.pone.0297014. eCollection 2024.
3
Telomerase-independent survival leads to a mosaic of complex subtelomere rearrangements in .
Genome Res. 2023 Sep;33(9):1582-1598. doi: 10.1101/gr.278043.123. Epub 2023 Aug 14.
4
Telomere-to-telomere assemblies of 142 strains characterize the genome structural landscape in Saccharomyces cerevisiae.
Nat Genet. 2023 Aug;55(8):1390-1399. doi: 10.1038/s41588-023-01459-y. Epub 2023 Jul 31.
5
Plant genome sequence assembly in the era of long reads: Progress, challenges and future directions.
Quant Plant Biol. 2022 Mar 11;3:e5. doi: 10.1017/qpb.2021.18. eCollection 2022.
6
Diverse Populations of Staphylococcus pseudintermedius Colonize the Skin of Healthy Dogs.
Microbiol Spectr. 2023 Feb 14;11(2):e0339322. doi: 10.1128/spectrum.03393-22.
8
A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis.
Plant Commun. 2023 Mar 13;4(2):100493. doi: 10.1016/j.xplc.2022.100493. Epub 2022 Nov 17.
10
Phylogenetic profiling resolves early emergence of PRC2 and illuminates its functional core.
Life Sci Alliance. 2022 Apr 19;5(7). doi: 10.26508/lsa.202101271. Print 2022 Jul.

本文引用的文献

2
BUSCO: Assessing Genome Assembly and Annotation Completeness.
Methods Mol Biol. 2019;1962:227-245. doi: 10.1007/978-1-4939-9173-0_14.
3
Canu: scalable and accurate long-read assembly via adaptive -mer weighting and repeat separation.
Genome Res. 2017 May;27(5):722-736. doi: 10.1101/gr.215087.116. Epub 2017 Mar 15.
4
Fast and accurate de novo genome assembly from long uncorrected reads.
Genome Res. 2017 May;27(5):737-746. doi: 10.1101/gr.214270.116. Epub 2017 Jan 18.
5
Whole-Genome Resequencing Reveals Extensive Natural Variation in the Model Green Alga Chlamydomonas reinhardtii.
Plant Cell. 2015 Sep;27(9):2353-69. doi: 10.1105/tpc.15.00492. Epub 2015 Sep 21.
7
Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement.
PLoS One. 2014 Nov 19;9(11):e112963. doi: 10.1371/journal.pone.0112963. eCollection 2014.
8
The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science. 2007 Oct 12;318(5848):245-50. doi: 10.1126/science.1143609.
9
Natural history of transposition in the green alga Chlamydomonas reinhardtii: use of the AMT4 locus as an experimental system.
Genetics. 2006 Aug;173(4):2005-19. doi: 10.1534/genetics.106.058263. Epub 2006 May 15.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验