Silva Ana Sanches, Brites Carla, Pouca Ana Vila, Barbosa Jorge, Freitas Andreia
National Institute for Agricultural and Veterinary Research (INIAV), Rua dos Lágidos, Lugar da Madalena, 4485-655 Vila do Conde, Portugal.
Center for Study in Animal Science (CECA), ICETA, University of Oporto, Oporto, Portugal.
Curr Res Food Sci. 2019 Jul 31;1:1-7. doi: 10.1016/j.crfs.2019.07.001. eCollection 2019 Nov.
An Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC-ToF-MS) method has been developed for determination of nine mycotoxins, namely aflatoxins (AFB, AFB, AFG and AFG), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and fumonisins (FB and FB) in maize. The method included a two-step extraction with acetonitrile 80% (v/v). After optimization, the analytical method was validated. The different concentrations tested take in account the Maximum Levels (ML) for maize (Commission Regulation EC no. 1881/2006) and good results for repeatability (%RSD ≤ 15.4%), reproducibility (%RSD ≤ 15.9%) and recovery (77.8-110.4%, except for AFG2 at 2 μg/kg which presented a recovery of 73.4%) were achieved. These met the performance criteria imposed by Commission Regulation (EC) no. 401/2006. The method was applied to twenty-two samples from Portuguese producers of maize. Fumonisins were the most frequently detected mycotoxins, but the levels do not exceed those imposed by European legislation.
已开发出一种超高效液相色谱与飞行时间质谱联用（UHPLC-ToF-MS）的方法，用于测定玉米中的九种霉菌毒素，即黄曲霉毒素（AFB₁、AFB₂、AFG₁和AFG₂）、赭曲霉毒素A（OTA）、玉米赤霉烯酮（ZEA）、T-2毒素（T-2）以及伏马毒素（FB₁和FB₂）。该方法包括用80%（v/v）乙腈进行两步萃取。优化后，对该分析方法进行了验证。所测试的不同浓度考虑了玉米的最大限量（欧盟委员会法规EC No. 1881/2006），并在重复性（%RSD≤15.4%）、再现性（%RSD≤15.9%）和回收率（77.8 - 110.4%，2 μg/kg的AFG₂除外，其回收率为73.4%）方面取得了良好结果。这些结果符合欧盟委员会法规（EC）No. 401/2006规定的性能标准。该方法应用于来自葡萄牙玉米生产商的22个样品。伏马毒素是最常检测到的霉菌毒素，但含量未超过欧洲法规规定的水平。
