Vahabi Surena, Nazemisalman Bahareh, Hosseinpour Sepanta, Salavitabar Simindokht, Aziz Alireza
Department of Periodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pedodontics, Zanjan University of Medical Sciences, Zanjan, Iran.
J Investig Clin Dent. 2018 May;9(2):e12319. doi: 10.1111/jicd.12319. Epub 2018 Feb 5.
Chronic periodontitis (CP) is a multifactorial disease and the most common type of periodontitis mainly caused by microbial plaque. CP can be brought on by, and progresses with, insufficient oral hygiene, and environmental and genetic susceptibilities. The aim of the present study was to investigate the association between interleukin (IL)-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) gene polymorphisms and the susceptibility to CP in an Iranian population.
Ninety-nine cases diagnosed with CP and 75 matched healthy controls engaged in the present study. 3 cc peripheral blood samples were obtained for DNA isolation. Genotype analysis was performed using restriction fragment length polymorphism polymerase chain reaction. Genotype distribution and allele frequencies within groups were compared using χ -test, and logistic regression analysis was used to recognize the independent relation between the disease and the absence or presence of alleles.
There was no polymorphism in IL-2 (T-330G) among our patients, and the TT genotype was present in both study groups. Moreover, none of the studied genotypes and alleles of IL-16 (T-295C) and IL-17 (A-7383G) was significantly associated with CP.
The present study demonstrated no association between IL-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) genotypes and CP in an Iranian population.
慢性牙周炎（CP）是一种多因素疾病，也是最常见的牙周炎类型，主要由微生物菌斑引起。CP可由口腔卫生不良、环境和遗传易感性引发并随之进展。本研究的目的是调查伊朗人群中白细胞介素（IL）-2（T-330G）、IL-16（T-295C）和IL-17（A-7383G）基因多态性与CP易感性之间的关联。
本研究纳入了99例诊断为CP的患者和75例匹配的健康对照。采集3毫升外周血样本用于DNA分离。使用限制性片段长度多态性聚合酶链反应进行基因型分析。采用χ检验比较各组内的基因型分布和等位基因频率，并使用逻辑回归分析来确定疾病与等位基因存在与否之间的独立关系。
我们的患者中IL-2（T-330G）没有多态性，两个研究组中均存在TT基因型。此外，IL-16（T-295C）和IL-17（A-7383G）的研究基因型和等位基因均与CP无显著关联。
本研究表明，在伊朗人群中，IL-2（T-330G）、IL-16（T-295C）和IL-17（A-7383G）基因型与CP之间无关联。
