Risoluti Roberta, Gullifa Giuseppina, Carcassi Elena, Masotti Andrea, Materazzi Stefano
Department of Chemistry, "Sapienza", University of Rome, p.le A.Moro 5, 00185, Rome, Italy.
Bambino Gesù Children's Hospital, IRCCS, Research Laboratories, v.le di San Paolo 15, 00146, Rome, Italy.
J Pharm Anal. 2020 Aug;10(4):351-355. doi: 10.1016/j.jpha.2020.02.009. Epub 2020 Feb 27.
In this work, functionalized carbon nanotubes (CNTs) using two polyamine polymers, polyethyleneimine (PEI) and polyamidoamine dendrimer (PAMAM), were investigated by thermal analysis in order to address preparation strategies to obtain low cytotoxic compounds with the ability to conjugate microRNAs and, at the same time, to transfect efficiently endothelial cells. Thermogravimetric analysis (TGA) was coupled to chemometrics as a novel analytical strategy to characterize functionalized CNTs from different preparation conditions. In particular, two starting materials were considered: very small CNTs and carboxylated CNTs (CNT-COOH) in order to examine the affinity with polymers. Chemometrics permitted to compare results from TGA and to investigate the effect of a number of factors affecting the synthesis of coated nanotubes including a different amount of involved polymer and the time required for the suspension for a satisfactory and reproducible preparation procedure. The results demonstrated the effectiveness of TGA as a tool able to address synthesis of coated CNTs to be employed as efficient drug delivery vectors in biomedical applications.
在这项工作中，通过热分析研究了使用两种多胺聚合物（聚乙烯亚胺（PEI）和聚酰胺胺树枝状大分子（PAMAM））功能化的碳纳米管（CNT），以探讨制备策略，从而获得具有结合微小RNA能力且同时能有效转染内皮细胞的低细胞毒性化合物。热重分析（TGA）与化学计量学相结合，作为一种新颖的分析策略，用于表征不同制备条件下的功能化碳纳米管。具体而言，考虑了两种起始材料：非常小的碳纳米管和羧基化碳纳米管（CNT-COOH），以研究与聚合物的亲和力。化学计量学有助于比较热重分析的结果，并研究影响包覆纳米管合成的多种因素的作用，这些因素包括不同用量的相关聚合物以及为获得令人满意且可重复的制备过程所需的悬浮时间。结果证明了热重分析作为一种工具的有效性，该工具能够指导用于生物医学应用中作为高效药物递送载体的包覆碳纳米管的合成。
