Laboratório de Pesquisa em Fármacos, Curso de Farmácia, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Rodovia Juscelino Kubitsheck, km 02, Macapá, Amapá, 68903-419, Brazil.
Programa de Pós-graduação em Inovação Farmacêutica, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Macapá, Brazil.
Inflammopharmacology. 2021 Feb;29(1):123-135. doi: 10.1007/s10787-020-00754-y. Epub 2020 Sep 14.
Bothrops moojeni snake venom (VBm) has toxins that cause pronounced tissue damage and exacerbated inflammatory reaction. Cannabis sativa L. is a plant species that produces an oil (CSO) rich in unsaturated fatty acids. Nano-emulsions have several advantages, such as better stability and higher penetrating power in membranes. Therefore, this study evaluated the effect of a nano-emulsion based on this herbal derivative (NCS) against VBm-induced inflammation in Wistar rats.
The CSO and NCS were submitted to physicochemical characterization. The inflammatory process was induced by the VBm (0.10 mg/kg) as follows: rat paw edema, peritonitis, analysis of leukocyte infiltrate in gastrocnemius muscle of rats and formation of granulomatous tissue.
No significant changes were observed when the NCS was submitted to the centrifugation and thermal stress tests. There was no phase separation, changes in density (0.978 ± 0.01 g/cm) and viscosity (0.889 ± 0.15). The droplet diameter ranged from 119.7 ± 065 to 129.3 ± 0.15 nm and the polydispersity index ranged from 0.22 ± 0.008 to 0.23 ± 0.011. The results showed that treatments with CSO (200 and 400 mg/kg) and NCS (100 mg/kg) were able to decrease significantly (p < 0.001) the formation of edema and granulomatous tissue. The CSO and NCS groups significantly attenuated (p < 0.001) the recruitment of inflammatory cells in the tests for peritonitis and leukocyte infiltrate. The histopathological analysis of the gastrocnemius muscle showed a reduction in tissue damage caused by VBm.
The results obtained in this study showed anti-inflammatory activity of the CSO which may be due to a high UFA content. The nanosizing, as evidenced by the incorporation of the CSO in the NCS improved the effect and opens the perspective for the obtainment of a nanomedicine in which a kinetic stable phytotherapic can be used at low doses.
矛头蝮蛇毒液（VBm）具有引起明显组织损伤和加重炎症反应的毒素。大麻 sativa L. 是一种产生富含不饱和脂肪酸油（CSO）的植物物种。纳米乳液具有许多优点，例如在膜中具有更好的稳定性和更高的穿透力。因此，本研究评估了基于这种草药衍生产品的纳米乳液（NCS）对 Wistar 大鼠 VBm 诱导的炎症的影响。
对 CSO 和 NCS 进行理化特性表征。通过 VBm（0.10mg/kg）诱导炎症过程：大鼠爪水肿、腹膜炎、大鼠腓肠肌白细胞浸润分析和肉芽肿组织形成。
NCS 经离心和热应力试验后未观察到明显变化。没有相分离、密度（0.978±0.01g/cm）和粘度（0.889±0.15）变化。液滴直径范围为 119.7±065 至 129.3±0.15nm，多分散指数范围为 0.22±0.008 至 0.23±0.011。结果表明，CSO（200 和 400mg/kg）和 NCS（100mg/kg）治疗能够显著降低（p<0.001）水肿和肉芽肿组织的形成。CSO 和 NCS 组在腹膜炎和白细胞浸润试验中显著减少（p<0.001）炎症细胞的募集。腓肠肌的组织病理学分析显示 VBm 引起的组织损伤减少。
本研究结果表明 CSO 具有抗炎活性，这可能是由于其高 UFA 含量。纳米化，如 CSO 被纳入 NCS 所证明的，改善了效果，并为获得一种动力学稳定的植物药开辟了前景，这种植物药可以以低剂量使用。
