College of Life Sciences, Northwest A&F University, Yangling, China.
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, China.
BMC Genomics. 2020 Sep 14;21(1):630. doi: 10.1186/s12864-020-07023-w.
The contribution of mitogen-activated protein kinase (MAPK) cascades to plant growth and development has been widely studied, but this knowledge has not yet been extended to the medicinal plant Salvia miltiorrhiza, which produces a number of pharmacologically active secondary metabolites.
In this study, we performed a genome-wide survey and identified six MAPKKK kinases (MAPKKKKs), 83 MAPKK kinases (MAPKKKs), nine MAPK kinases (MAPKKs) and 18 MAPKs in the S. miltiorrhiza genome. Within each class of genes, a small number of subfamilies were recognized. A transcriptional analysis revealed differences in the genes' behaviour with respect to both their site of transcription and their inducibility by elicitors and phytohormones. Two genes were identified as strong candidates for playing roles in phytohormone signalling. A gene-to-metabolite network was constructed based on correlation analysis, highlighting the likely involvement of two of the cascades in the synthesis of two key groups of pharmacologically active secondary metabolites: phenolic acids and tanshinones.
The data provide insight into the functional diversification and conservation of MAPK cascades in S. miltiorrhiza.
丝裂原活化蛋白激酶（MAPK）级联在植物生长发育中的作用已得到广泛研究，但这一知识尚未扩展到药用植物丹参，丹参产生多种具有药理活性的次生代谢产物。
本研究在丹参基因组中进行了全基因组调查，鉴定出 6 个丝裂原活化蛋白激酶激酶激酶（MAPKKKKs）、83 个丝裂原活化蛋白激酶激酶（MAPKKKs）、9 个丝裂原活化蛋白激酶激酶（MAPKKs）和 18 个丝裂原活化蛋白激酶（MAPKs）。在每一类基因中，都识别出少量的亚家族。转录分析显示，基因的行为在转录部位和对诱导子和植物激素的诱导方面存在差异。鉴定出两个基因作为在植物激素信号转导中发挥作用的强候选基因。根据相关分析构建了基因-代谢物网络，突出了两个级联在合成两类具有药理活性的次生代谢产物：酚酸和丹参酮中的可能参与。
这些数据为 MAPK 级联在丹参中的功能多样化和保守性提供了深入了解。
