Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Yogyakarta Special Region, Indonesia;, Email:
Laboratorium of Anatomic Pathology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.
J Am Assoc Lab Anim Sci. 2020 Nov 1;59(6):673-680. doi: 10.30802/AALAS-JAALAS-20-000013. Epub 2020 Sep 14.
Several diagnostic tools are available for veterinarians and fish health professionals to evaluate fish health and their abnormalities. However, reference data regarding the character and size of fish blood cells are limited. The purpose of this study was to analyze the morphology and morphometry of normal blood cells in zebrafish (), common carp (), and tilapia (). To get a representative sample, we took blood from ten 6-mo old healthy fish from each species. Fish were purchased from an ornamental fish market and the local government fish breeding center in Yogyakarta, Indonesia. A total of 100 erythrocytes and a maximum of 30 leukocytes (neutrophils, eosinophils, basophils, lymphocytes and monocytes) were randomly sampled and analyzed qualitatively and quantitatively, including morphometric analysis of both the long axis (LA) and short axis (SA) of these cells. All data obtained were analyzed using ANOVA statistical tests to compare the blood cells in each species with SPSS software. The findings revealed distinct differences in both morphology and morphometry of the blood cells among the species. Basic knowledge obtained from this research will aid in the development of biomarkers and other ancillary diagnostic tools for further hematology research, conservation, and clinical diagnosis in these 3 fish species.
有几种诊断工具可供兽医和鱼类健康专家用于评估鱼类健康及其异常情况。然而，有关鱼类血细胞特征和大小的参考数据有限。本研究的目的是分析斑马鱼（）、鲤鱼（）和罗非鱼（）正常血细胞的形态和形态计量学。为了获得有代表性的样本，我们从每个物种的 10 只 6 月龄的健康鱼中抽取血液。这些鱼是从印度尼西亚日惹的观赏鱼市场和当地政府鱼苗繁殖中心购买的。总共随机抽取了 100 个红细胞和最多 30 个白细胞（中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、淋巴细胞和单核细胞），对这些细胞的长轴（LA）和短轴（SA）进行定性和定量分析，包括形态计量分析。使用 SPSS 软件对所有获得的数据进行方差分析统计检验，以比较每个物种的血细胞。研究结果表明，这 3 种鱼类的血细胞在形态和形态计量学上存在明显差异。从这项研究中获得的基本知识将有助于为这 3 种鱼类的进一步血液学研究、保护和临床诊断开发生物标志物和其他辅助诊断工具。
