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从幼仓鼠肾（BHK - 21）细胞中分离出10纳米细丝并进行初步表征。

Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.

作者信息

Starger J M, Goldman R D

出版信息

Proc Natl Acad Sci U S A. 1977 Jun;74(6):2422-6. doi: 10.1073/pnas.74.6.2422.

DOI:10.1073/pnas.74.6.2422
PMID:329284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432184/
Abstract

A procedure for isolating 10-nm filaments as juxtanuclear caps from normal and colchicine-treated BHK-21 cells is described. These aggregates of 10-nm filaments retain their birefringence and their structural integrity. The major proteins comprising the filament preparations can be resolved as two bands on sodium dodecyl sulfate gels, corresponding to approximate molecular weights of 54,000 and 55,000.

摘要

本文描述了一种从正常和秋水仙碱处理的BHK - 21细胞中分离10纳米细丝作为近核帽的方法。这些10纳米细丝的聚集体保持其双折射和结构完整性。组成细丝制剂的主要蛋白质在十二烷基硫酸钠凝胶上可解析为两条带，分别对应于约54,000和55,000的分子量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/3c23ccf8e7e0/pnas00028-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/79ed47c04cc2/pnas00028-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/3d4278df1478/pnas00028-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/e0082cfa84c0/pnas00028-0242-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/314a0ce171aa/pnas00028-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/3c23ccf8e7e0/pnas00028-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/79ed47c04cc2/pnas00028-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/3d4278df1478/pnas00028-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/e0082cfa84c0/pnas00028-0242-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/314a0ce171aa/pnas00028-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47e4/432184/3c23ccf8e7e0/pnas00028-0243-b.jpg

相似文献

1
Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.从幼仓鼠肾（BHK - 21）细胞中分离出10纳米细丝并进行初步表征。
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2422-6. doi: 10.1073/pnas.74.6.2422.
2
Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells.对从幼仓鼠肾（BHK-21）细胞中快速纯化的10纳米细丝进行生化和免疫分析。
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3
The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.三种细胞质纤维在BHK - 21细胞运动中的作用。I. 微管及秋水仙碱的作用
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Dynamic properties of intermediate filaments: disassembly and reassembly during mitosis in baby hamster kidney cells.中间丝的动态特性：在幼仓鼠肾细胞有丝分裂期间的解聚与重新组装
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Science. 1970 Jul 17;169(3942):286-8. doi: 10.1126/science.169.3942.286.
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A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS.一种用于蛋白质定量的微量双缩脲法。
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Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae.绘制生殖支原体和肺炎支原体中的磷酸化蛋白质图谱。
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J Cell Biol. 1971 Dec;51(3):752-62. doi: 10.1083/jcb.51.3.752.
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Science. 1970 Jul 17;169(3942):286-8. doi: 10.1126/science.169.3942.286.