从幼仓鼠肾（BHK - 21）细胞中分离出10纳米细丝并进行初步表征。
Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.
作者信息
Starger J M, Goldman R D
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2422-6. doi: 10.1073/pnas.74.6.2422.
Abstract
A procedure for isolating 10-nm filaments as juxtanuclear caps from normal and colchicine-treated BHK-21 cells is described. These aggregates of 10-nm filaments retain their birefringence and their structural integrity. The major proteins comprising the filament preparations can be resolved as two bands on sodium dodecyl sulfate gels, corresponding to approximate molecular weights of 54,000 and 55,000.
摘要
本文描述了一种从正常和秋水仙碱处理的BHK - 21细胞中分离10纳米细丝作为近核帽的方法。这些10纳米细丝的聚集体保持其双折射和结构完整性。组成细丝制剂的主要蛋白质在十二烷基硫酸钠凝胶上可解析为两条带，分别对应于约54,000和55,000的分子量。
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