Han Yu, Lian Meifei, Sun Binbin, Jia Bo, Wu Qiang, Qiao Zhiguang, Dai Kerong
Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Theranostics. 2020 Aug 13;10(22):10214-10230. doi: 10.7150/thno.47909. eCollection 2020.
Articular cartilage injury is quite common. However, post-injury cartilage repair is challenging and often requires medical intervention, which can be aided by 3D printed tissue engineering scaffolds. Specifically, the high accuracy of Melt Electro-Writing (MEW) technology facilitates the printing of scaffolds that imitate the structure and composition of natural cartilage to promote repair. MEW and Inkjet printing technology was employed to manufacture a composite scaffold that was then implanted into a cartilage injury site through microfracture surgery. While printing polycaprolactone (PCL) or PCL/hydroxyapatite (HA) scaffolds, cytokine-containing microspheres were sprayed alternately to form multiple layers containing transforming growth factor-β1 and bone morphogenetic protein-7 (surface layer), insulin-like growth factor-1 (middle layer), and HA (deep layer). The composite biological scaffold was conducive to adhesion, proliferation, and differentiation of mesenchymal stem cells recruited from the bone marrow and blood. Meanwhile, the environmental differences between the scaffold's layers contributed to the regional heterogeneity of chondrocytes and secreted proteins to promote functional cartilage regeneration. The biological effect of the composite scaffold was validated both and . A cartilage repair scaffold was established with high precision as well as promising mechanical and biological properties. This scaffold can promote the repair of cartilage injury by using, and inducing the differentiation and expression of, autologous bone marrow mesenchymal stem cells.
关节软骨损伤十分常见。然而，损伤后软骨修复具有挑战性，通常需要医学干预，而3D打印组织工程支架对此可能有所帮助。具体而言，熔体静电纺丝（MEW）技术的高精度有助于打印模仿天然软骨结构和组成的支架以促进修复。采用MEW和喷墨打印技术制造了一种复合支架，然后通过微骨折手术将其植入软骨损伤部位。在打印聚己内酯（PCL）或PCL/羟基磷灰石（HA）支架时，交替喷涂含细胞因子的微球以形成包含转化生长因子-β1和骨形态发生蛋白-7（表层）、胰岛素样生长因子-1（中层）和HA（深层）的多层结构。该复合生物支架有利于从骨髓和血液中募集的间充质干细胞的黏附、增殖和分化。同时，支架各层之间的环境差异导致软骨细胞和分泌蛋白的区域异质性，从而促进功能性软骨再生。复合支架的生物学效应在[此处原文缺失验证情况的具体内容]均得到了验证。构建了一种具有高精度以及良好力学和生物学性能的软骨修复支架。该支架可通过利用并诱导自体骨髓间充质干细胞的分化和表达来促进软骨损伤的修复。
