水样液缺乏性干眼症患者的眼表微生物组。

Ocular surface microbiota in patients with aqueous tear-deficient dry eye.

机构信息

Department of Ophthalmology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

出版信息

Ocul Surf. 2021 Jan;19:210-217. doi: 10.1016/j.jtos.2020.09.003. Epub 2020 Sep 12.

DOI:10.1016/j.jtos.2020.09.003

PMID:32931939

Abstract

PURPOSE

An altered ocular surface microbiota may contribute to the pathophysiology of dry eye disease. The aim of the study was to explore potential differences in microbiota diversity and composition in aqueous tear-deficient dry eye (with and without ocular graft-versus-host disease) compared with controls.

METHODS

Swab samples from the inferior fornix of the conjunctiva were obtained from patients with aqueous tear-deficient dry eye with and without ocular graft-versus-host disease (n = 18, n = 21, respectively) and controls (n = 28). Isolated bacterial DNA from swabs were analyzed with 16S rRNA gene amplicon sequencing.

RESULTS

Decreased microbiota diversity was observed in patients with aqueous tear-deficient dry eye (p ≤ 0.003) who also showed a difference in microbiota composition compared with controls (p = 0.001). Although several genera were less abundant in aqueous tear-deficient dry eye, a minimal core ocular surface microbiota comprising five genera was shared by >75% of the study participants: Enhydrobacter, Brevibacterium, Staphylococcus, Streptococcus and Cutibacterium. Pseudomonas was identified as a bacterial biomarker for controls and Bacilli for patients with aqueous tear-deficient dry eye.

CONCLUSIONS

Ocular surface microbiota in patients with aqueous tear-deficient dry eye was characterized by an aberrant microbiota composition in comparison to controls, with decreased diversity and reduced relative abundances of several genera. Additionally, a few genera were present in most of the study population, indicating that a minimal core ocular surface microbiota may exist.

摘要

目的

眼表微生物群的改变可能有助于干燥性眼病的病理生理学。本研究旨在探索与对照组相比，水样液缺乏性干眼（伴或不伴眼移植物抗宿主病）的眼表微生物多样性和组成的潜在差异。

方法

从水样液缺乏性干眼伴眼移植物抗宿主病（n=18，n=21）和对照组（n=28）患者的下穹窿结膜拭子中获得结膜下穹窿拭子样本。用 16S rRNA 基因扩增子测序分析拭子中分离的细菌 DNA。

结果

与对照组相比，水样液缺乏性干眼患者的微生物多样性降低（p≤0.003），且其微生物组成也存在差异（p=0.001）。尽管水样液缺乏性干眼患者的几种菌属数量较少，但>75%的研究参与者都存在由五个菌属组成的最小核心眼表微生物群： Enhydrobacter、Brevibacterium、Staphylococcus、Streptococcus 和 Cutibacterium。假单胞菌被鉴定为对照组的细菌生物标志物，而杆菌则为水样液缺乏性干眼患者的细菌生物标志物。