Guo Junda, Huang Zhen, Sun Jialing, Cui Xiuming, Liu Yuan
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Yuxi Walvax Biotechnology Co., Ltd., Yuxi, China.
Front Plant Sci. 2021 Jul 28;12:691838. doi: 10.3389/fpls.2021.691838. eCollection 2021.
Transcriptomics is one of the most popular topics in biology in recent times. Transcriptome sequencing (RNA-Seq) is a high-throughput, high-sensitivity, and high-resolution technique that can be used to study model and non-model organisms. Transcriptome sequencing is also an important method for studying the genomes of medicinal plants, a topic on which limited information is available. The study of medicinal plants through transcriptomics can help researchers analyze functional genes and regulatory mechanisms of medicinal plants and improve breeding selection and cultivation techniques. This article analyzes and compares the applications of transcriptome sequencing in medicinal plants over the past decade and briefly introduces the methods of transcriptome sequencing and analysis, their applications in medicinal plant research, and potential development trends. We will focus on the research and application progress of transcriptome sequencing in the following four areas: the mining of functional genes in medicinal plants, development of molecular markers, biosynthetic pathways of secondary metabolites, and developmental mechanisms of medicinal plants. Our review will provide ideas for the mining of functional genes of medicinal plants and breeding new varieties.
转录组学是近年来生物学领域最热门的话题之一。转录组测序（RNA-Seq）是一种高通量、高灵敏度和高分辨率的技术，可用于研究模式生物和非模式生物。转录组测序也是研究药用植物基因组的重要方法，而关于这一主题的信息有限。通过转录组学研究药用植物可以帮助研究人员分析药用植物的功能基因和调控机制，改进育种选择和栽培技术。本文分析并比较了过去十年中转录组测序在药用植物中的应用，并简要介绍了转录组测序与分析方法、它们在药用植物研究中的应用以及潜在的发展趋势。我们将重点关注转录组测序在以下四个领域的研究与应用进展：药用植物功能基因挖掘、分子标记开发、次生代谢物生物合成途径以及药用植物发育机制。我们的综述将为药用植物功能基因挖掘和新品种培育提供思路。
