Karlsson-Parra A, Kobbah M, Ewald U, Tuvemo T, Forsum U, Klareskog L
Department of Clinical Bacteriology, University of Uppsala, Sweden.
Acta Paediatr Scand. 1988 Jul;77(4):554-8. doi: 10.1111/j.1651-2227.1988.tb10699.x.
The occurrence of circulating class II antigen-expressing T lymphocytes was examined in 25 children with insulin-dependent diabetes mellitus using an indirect double immunofluorescence assay on prefixed cell samples. In order to exclude sensitization to heterologous insulin as a possible factor affecting the results, the patients were investigated at the day of clinical onset, before receiving insulin. An increased percentage of T cells (CD3+ cells) expressing class II antigens was seen in 19 out of 25 patients and class II expression was observed on cells within both the CD4+ and CD8+ T cell subsets. No correlation was found between the levels of class II expressing T cells and the individual degree of metabolic derangement or indicators of recent infection. Re-testing of 16 patients after one year on insulin treatment revealed a significant decrease of class II expressing T cell levels. Our data suggest that the increased levels of class II expressing T cells seen in IDDM of recent onset directly reflects immune reactions that are related to the disease process.
采用间接双免疫荧光法对25例胰岛素依赖型糖尿病患儿的预固定细胞样本进行检测，以观察循环中表达II类抗原的T淋巴细胞的出现情况。为排除对异源胰岛素致敏这一可能影响结果的因素，在临床发病当天、未接受胰岛素治疗前对患者进行了研究。25例患者中有19例出现表达II类抗原的T细胞（CD3+细胞）百分比增加，且在CD4+和CD8+ T细胞亚群的细胞上均观察到II类抗原表达。未发现表达II类抗原的T细胞水平与个体代谢紊乱程度或近期感染指标之间存在相关性。对16例接受胰岛素治疗一年后的患者进行重新检测，结果显示表达II类抗原的T细胞水平显著下降。我们的数据表明，近期发病的胰岛素依赖型糖尿病患者中表达II类抗原的T细胞水平升高直接反映了与疾病进程相关的免疫反应。
