Ahmed S, Sochor M, Tabidi I, McLean P
The Department of Biochemistry, University College, London.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):443-9. doi: 10.1016/0006-291x(88)90706-1.
The effects of insulin and proinsulin have been measured on the rates of glucose oxidation via the pentose phosphate pathway and incorporation into lipid in slices of lactating rat mammary gland. Half-maximal stimulation of glucose oxidation was observed with 1-3 x 10(-8)M insulin while 1 x 10(-7)M proinsulin was required to achieve half-maximal stimulation. A similar, approximately 10-fold, difference in potency was observed in relation to lipid synthesis. The present results appear to indicate that the maximum stimulation of either glucose oxidation via the pentose phosphate pathway or lipid synthesis by proinsulin did not reach the same level as that found for insulin.
已经测定了胰岛素和胰岛素原对泌乳大鼠乳腺切片中通过磷酸戊糖途径的葡萄糖氧化速率以及葡萄糖掺入脂质的速率的影响。观察到1-3×10⁻⁸M胰岛素可使葡萄糖氧化达到半最大刺激，而需要1×10⁻⁷M胰岛素原才能达到半最大刺激。在脂质合成方面也观察到了类似的、约10倍的效价差异。目前的结果似乎表明，胰岛素原对通过磷酸戊糖途径的葡萄糖氧化或脂质合成的最大刺激未达到与胰岛素相同的水平。
