Department of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of Korea.
Institute for Biomaterials, Korea University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5640-5647. doi: 10.1099/ijsem.0.004455. Epub 2020 Sep 16.
Three bacterial strains, namely HYN0069, HYN0085 and HYN0086, were isolated from freshwater samples taken from the Namhangan River system in Korea. 16S rRNA gene sequence similarities and phylogenetic tree topologies indicated that the three strains belonged to the genera , and by showing the highest sequence similarities with (98.4 %), (98.3 %) and (98.1 %). No bacterial species with validly published names showed 98.7 % or higher sequence similarity with the novel isolates. The average nucleotide identities between the genome sequences of the three new isolates and the three closest neighbours were 80.2-92.0 %, all below the threshold for bacterial species delineation (95-96 %). Many biochemical and physiological features also discriminated the isolates from previously known species of the genera , and . Based on the phylogenetic and phenotypic data presented in this study, we suggest three novel species with the following names: sp. nov. (type strain HYN0069=KACC 19488=NBRC 113115), sp. nov. (type strain HYN0085=KACC 19490=NBRC 113116) and sp. nov. (type strain HYN0086=KACC 19489=NBRC 113117).
三株细菌菌株，即 HYN0069、HYN0085 和 HYN0086，从韩国南汉江系统的淡水样本中分离得到。16S rRNA 基因序列相似性和系统发育树拓扑结构表明，这三株菌属于 、 和 属，与 （98.4%）、 （98.3%）和 （98.1%）的序列相似度最高。没有具有有效发表名称的细菌种与新分离株的相似度达到 98.7%或更高。三个新分离株与三个最接近的近缘种的全基因组序列之间的平均核苷酸同一性为 80.2-92.0%，均低于细菌种划分的阈值（95-96%）。许多生化和生理特征也将这些分离株与 、 和 属中以前已知的种区分开来。根据本研究中提供的系统发育和表型数据，我们建议将这三个新种命名为： sp. nov.（模式菌株 HYN0069=KACC 19488=NBRC 113115）、 sp. nov.（模式菌株 HYN0085=KACC 19490=NBRC 113116）和 sp. nov.（模式菌株 HYN0086=KACC 19489=NBRC 113117）。
