Baek Chaeyun, Shin Su-Kyoung, Yi Hana
1Department of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of Korea.
2School of Biosystem and Biomedical Science, Korea University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3837-3843. doi: 10.1099/ijsem.0.003067. Epub 2018 Oct 15.
Four new limnic bacterial strains, namely HYN0048, HYN0049, HYN0056 and HYN0059, were isolated from two lakes in the Nakdong River water system in Korea. 16S rRNA gene sequence analyses indicated that strains HYN0048, HYN0049, HYN0056 and HYN0059 belonged to the genus Flavobacterium by showing the highest sequence similarities with the type strains of F. soli (94.9 %), F. keumense (95.1 %), F. dispersum (97.7 %) and F. hauense (97.6 %), respectively. The low sequence similarities and tree topologies implied the novelty of the four isolates, as novel genomic species of the genus Flavobacterium. Numerous physiological and biochemical features also supported the distinctiveness of the isolates from previously known bacterial species. On the basis of the phylogenetic and phenotypic data presented in this study, four novel species are suggested with the following names: Flavobacterium magnum sp. nov. (type strain HYN0048=KACC 19180=NBRC 112740), Flavobacterium pallidum sp. nov. (HYN0049=KACC 19181=NBRC 112741), Flavobacterium crocinum sp. nov. (HYN0056=KACC 19182=NBRC 112743) and Flavobacterium album sp. nov. (HYN0059=KACC 19183=NBRC 112744).
从韩国洛东江流域的两个湖泊中分离出了四株新的淡水细菌菌株，分别为HYN0048、HYN0049、HYN0056和HYN0059。16S rRNA基因序列分析表明，菌株HYN0048、HYN0049、HYN0056和HYN0059分别与土壤黄杆菌（94.9%）、锦江黄杆菌（95.1%）、分散黄杆菌（97.7%）和河原黄杆菌（97.6%）的模式菌株显示出最高的序列相似性，属于黄杆菌属。低序列相似性和系统发育树拓扑结构表明这四株分离株具有新颖性，是黄杆菌属的新基因组种。许多生理生化特征也支持这些分离株与先前已知细菌物种的差异性。基于本研究中呈现的系统发育和表型数据，建议将这四个新物种命名如下：大形黄杆菌（Flavobacterium magnum sp. nov.）（模式菌株HYN0048 = KACC 19180 = NBRC 112740）、苍白黄杆菌（Flavobacterium pallidum sp. nov.）（HYN0049 = KACC 19181 = NBRC 112741）、藏红花黄杆菌（Flavobacterium crocinum sp. nov.）（HYN0056 = KACC 19182 = NBRC 112743）和白色黄杆菌（Flavobacterium album sp. nov.）（HYN0059 = KACC 19183 = NBRC 112744）。
