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胶带剥离法可用于定量测定人类皮肤表面（包括角质层）上的抗菌肽。

Tape stripping method is useful for the quantification of antimicrobial peptides on the human skin surface including the stratum corneum.

机构信息

Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi, 321-3497, Japan.

Japan Institute of Sports Sciences, Tokyo, Japan.

出版信息

Sci Rep. 2020 Sep 17;10(1):15259. doi: 10.1038/s41598-020-72111-6.

DOI:10.1038/s41598-020-72111-6
PMID:32943667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7499253/
Abstract

Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

摘要

抗菌肽（AMPs）在人体皮肤的先天免疫中发挥着重要作用。已知 AMPs 主要在角质层中发挥作用。因此，需要精确测量角质层中的 AMP 浓度，以阐明 AMP 在皮肤防御中的功能和生理重要性。胶带剥离（TS）是一种成熟的方法，可以收集角质层中的成分。然而，TS 方法测量人体皮肤 AMP 浓度的有用性尚不清楚。因此，我们将其与另一种常用的方法，皮肤冲洗法进行了比较，皮肤冲洗法已被确立为测量人体皮肤 AMP 浓度的方法。当使用 RNase 7（一种典型的 AMP）作为指标在健康的中前臂内侧进行研究时，相邻前臂部位 TS 法测量的 RNase 7 浓度之间存在显著正相关，证明了 TS 法的可重复性。接下来，在使用 TS 和皮肤冲洗法测量的 RNase 7 浓度之间检测到显著正相关，表明 TS 法可与皮肤冲洗法相媲美。因此，我们推测 TS 法可用于测量人体皮肤中的 AMP 浓度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/ead8b947c3db/41598_2020_72111_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/efc5eca275d1/41598_2020_72111_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/57f6aaa934dc/41598_2020_72111_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/da13db08c83c/41598_2020_72111_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/30e5ac380eb4/41598_2020_72111_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/df96b8b7a150/41598_2020_72111_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/ead8b947c3db/41598_2020_72111_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/efc5eca275d1/41598_2020_72111_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/78162b8d3f90/41598_2020_72111_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/57f6aaa934dc/41598_2020_72111_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/30e5ac380eb4/41598_2020_72111_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/df96b8b7a150/41598_2020_72111_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d43/7499253/ead8b947c3db/41598_2020_72111_Fig7_HTML.jpg

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