Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.
College of Light Industry and Textile, Qiqihar University, Qiqihar, Heilongjiang 161000, People's Republic of China.
Biofabrication. 2020 Sep 18;12(4):045027. doi: 10.1088/1758-5090/abb11e.
We report a method for expanding microchannel-embedded paper devices using a precisely controlled gas-foaming technique for the generation of volumetric tissue models in vitro. We successfully fabricated hollow, perfusable microchannel patterns contained in a densely entangled network of bacterial cellulose nanofibrils using matrix-assisted sacrificial three-dimensional printing, and demonstrated the maintenance of their structural integrity after gas-foaming-enabled expansion in an aqueous solution of NaBH. The resulting expanded microchannel-embedded paper devices showed multilayered laminar structures with controllable thicknesses as a function of both NaBH concentration and expansion time. With expansion, the thickness and porosity of the bacterial cellulose network were significantly increased. As such, cellular infiltration was promoted comparing to as-prepared, non-expanded devices. This simple technique enables the generation of truly volumetric, cost-effective human-based tissue models, such as vascularized tumor models, for potential applications in preclinical drug screening and personalized therapeutic selection.
我们报告了一种使用精确控制的气体发泡技术扩展微通道嵌入式纸设备的方法，用于体外生成体积组织模型。我们成功地使用基质辅助牺牲三维打印制造了空心、可灌注的微通道图案，这些图案包含在细菌纤维素纳米纤维的密集缠结网络中，并证明了它们在 NaBH 的水溶液中进行气体发泡扩展后的结构完整性得以保持。所得的扩展微通道嵌入式纸设备显示出具有多层层状结构，其厚度可以通过 NaBH 浓度和扩展时间来控制。随着扩展，细菌纤维素网络的厚度和孔隙率显著增加。因此，与未经扩展的原始设备相比，细胞渗透得到了促进。这种简单的技术可以生成真正的体积、经济高效的基于人体的组织模型，例如血管化肿瘤模型，用于临床前药物筛选和个性化治疗选择的潜在应用。
