J Craig Venter Institute, Medical Center Drive, Rockville, MD, United States of America.
Department of Medicine, University of Pittsburgh, Pittsburgh, PA, United States of America.
PLoS One. 2020 Sep 18;15(9):e0237493. doi: 10.1371/journal.pone.0237493. eCollection 2020.
The phyllosphere epiphytic microbiome is composed of microorganisms that colonize the external aerial portions of plants. Relationships of plant responses to specific microorganisms-both pathogenic and beneficial-have been examined, but the phyllosphere microbiome functional and metabolic profile responses are not well described. Changing crop growth conditions, such as increased drought, can have profound impacts on crop productivity. Also, epiphytic microbial communities provide a new target for crop yield optimization. We compared Zea mays leaf microbiomes collected under drought and well-watered conditions by examining functional gene annotation patterns across three physically disparate locations each with and without drought treatment, through the application of short read metagenomic sequencing. Drought samples exhibited different functional sequence compositions at each of the three field sites. Maize phyllosphere functional profiles revealed a wide variety of metabolic and regulatory processes that differed in drought and normal water conditions and provide key baseline information for future selective breeding.
叶际附生微生物组由定植在植物外部气生部分的微生物组成。已经研究了植物对特定微生物（包括病原微生物和有益微生物）的反应关系，但叶际微生物组的功能和代谢特征反应还没有很好地描述。改变作物的生长条件，如增加干旱，会对作物的生产力产生深远的影响。此外，附生微生物群落为优化作物产量提供了一个新的目标。我们通过应用短读宏基因组测序，在三个物理上不同的地点，每个地点都有和没有干旱处理，比较了在干旱和充分浇水条件下收集的玉米叶片微生物组，通过检查功能基因注释模式。干旱样本在三个野外地点的每个地点都表现出不同的功能序列组成。玉米叶际功能图谱揭示了广泛的代谢和调控过程，这些过程在干旱和正常水分条件下有所不同，为未来的选择性育种提供了关键的基线信息。
