Burgoyne R D, Gray E G, Sullivan K, Barron J
Neurosci Lett. 1982 Jul 20;31(1):81-5. doi: 10.1016/0304-3940(82)90058-1.
Electron microscopical examination indicated that incubation of slices of rat cerebral cortex in Krebs buffer at room temperature of 37 degrees C led to a rapid and more or less complete depolymerization of dendritic microtubules. The loss of dendritic microtubules did not appear to be a consequence of anoxia. Myelinated axons showed only a partial loss of microtubules and the microtubules of preterminal axons were unaffected by incubation. These results indicate differential labilities of axonal and dendritic microtubules under these conditions of incubation. Such an effect of the incubation of slices in Krebs buffer indicates a need for caution in the interpretation of experiments on slice preparations.
电子显微镜检查表明，将大鼠大脑皮层切片在37摄氏度的 Krebs 缓冲液中于室温下孵育，会导致树突微管迅速且或多或少完全解聚。树突微管的丧失似乎并非缺氧所致。有髓轴突仅显示微管部分丧失，而终末前轴突的微管在孵育过程中未受影响。这些结果表明在这些孵育条件下轴突和树突微管的稳定性存在差异。在Krebs缓冲液中孵育切片的这种效应表明，在解释切片制备实验时需要谨慎。
