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两种兰花中R2R3-MYB转录因子基因的全基因组鉴定与表达分析

Genome-wide identification and expression analyses of R2R3-MYB transcription factor genes from two Orchid species.

作者信息

Fan Honghong, Cui Manli, Li Ninghong, Li Xujuan, Liang Yuxuan, Liu Lin, Cai Yongping, Lin Yi

机构信息

School of Life Sciences, Anhui Agricultural University, Hefei, China.

Faculty of Forestry, University of British Columbia, Vancouver, Canada.

出版信息

PeerJ. 2020 Sep 1;8:e9781. doi: 10.7717/peerj.9781. eCollection 2020.

Abstract

MYB transcription factors play important roles in different plant biological processes during plant growth, development and stress response. In this study, 101 () and 99 () genes were identified in the genomes of and , respectively. To classify the isolated candidate genes, the genes from were selected as references. As a result, all identified and genes were classified into 22 subfamilies. Phylogenetic analysis revealed that S21 had the largest number of members of all the subfamilies. The numbers of introns, exons and conserved sequences in all of the identified genes are different. In addition, 20 genes from six subfamilies were selected for further analysis of tissue-specific expression and responses to various abiotic stresses treatments. The results showed that all of the genes in S4 and S19 subfamilies exhibited the highest relative expression levels in flowers. And five genes including , , DoMYB49, and , responded to the stress response. These results may provide useful information for further studies of the R2R3-MYB gene family.

摘要

MYB转录因子在植物生长、发育和应激反应的不同植物生物学过程中发挥着重要作用。在本研究中,分别在和的基因组中鉴定出101个()和99个()基因。为了对分离出的候选基因进行分类,选择来自的基因作为参考。结果,所有鉴定出的和基因被分为22个亚家族。系统发育分析表明,S21在所有亚家族中成员数量最多。所有鉴定出的基因中的内含子、外显子和保守序列的数量各不相同。此外,从六个亚家族中选择了20个基因进行组织特异性表达和对各种非生物胁迫处理反应的进一步分析。结果表明,S4和S19亚家族中的所有基因在花中表现出最高的相对表达水平。包括、、DoMYB49、和在内的五个基因对胁迫反应有响应。这些结果可能为R2R3 - MYB基因家族的进一步研究提供有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8816/7473048/0d0228e57b14/peerj-08-9781-g001.jpg

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