褪黑素与注射用富血小板纤维蛋白对糖尿病大鼠下颌下唾液腺严重伤口愈合的影响
Effect of melatonin versus injectable platelet rich fibrin on critical wound healing in submandibular salivary glands of diabetic rats.
作者信息
Elsherbini Amira M, Ezzat Samah K
机构信息
Oral Biology Department, Faculty of Dentistry, Mansoura University, Egypt.
出版信息
J Oral Biol Craniofac Res. 2020 Oct-Dec;10(4):592-596. doi: 10.1016/j.jobcr.2020.08.016. Epub 2020 Aug 26.
BACKGROUND
Diabetes mellitus (DM) has harmful effects on body organs, including submandibular salivary glands (SMGs). It impairs wound healing process that follow sialoadenectomy. Yet there is no complete cure to diabetes, the available medications tend to control the side effects of DM or manage insulin resistance. Herein we tried to investigate the possible effects of injectable platelet rich fibrin (i-PRF) and melatonin on wound healing in diabetic rats.
MATERIAL AND METHODS
Surgical defects were created in SMGs of 30 rats after confirmation of DM induction. Then rats were randomly and equally allocated into three groups. Group I served as control group; group II received topically applied i-PRF, and group III received topically administrated melatonin. After 28 days all rats were euthanized, and SMGs were processed for histological and biochemical analysis.
RESULTS
Both i-PRF and melatonin caused significant reduction of malondialdehyde (P < 0.0001) and caspase-3 (P < 0.001) and significant increase in vascular endothelial growth factors (P = 0.001,0.009 respectively) that increased SMGs regenerative capacity when compared to diabetic group. Melatonin showed superior results regarding the histomorphological structure of SMGs.
CONCLUSION
Melatonin and i-PRF can be possible candidates for improvement of wound healing events in SMGs of diabetic rats.
背景
糖尿病（DM）对包括下颌下唾液腺（SMGs）在内的身体器官具有有害影响。它会损害涎腺切除术后的伤口愈合过程。然而，糖尿病尚无完全治愈方法，现有药物往往用于控制糖尿病的副作用或管理胰岛素抵抗。在此，我们试图研究注射用富血小板纤维蛋白（i-PRF）和褪黑素对糖尿病大鼠伤口愈合的可能影响。
材料与方法
在确认诱导糖尿病后，在30只大鼠的下颌下唾液腺制造手术缺损。然后将大鼠随机且平均分为三组。第一组作为对照组；第二组局部应用i-PRF，第三组局部给予褪黑素。28天后，对所有大鼠实施安乐死，并对下颌下唾液腺进行组织学和生化分析。
结果
与糖尿病组相比，i-PRF和褪黑素均导致丙二醛显著降低（P < 0.0001）和半胱天冬酶-3显著降低（P < 0.001），并使血管内皮生长因子显著增加（分别为P = 0.001、0.009），从而提高了下颌下唾液腺的再生能力。褪黑素在下颌下唾液腺的组织形态结构方面显示出更好的结果。
结论
褪黑素和i-PRF可能是改善糖尿病大鼠下颌下唾液腺伤口愈合情况的候选药物。
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