Mirzaei Farzaneh, Bafghi Ali Fattahi, Mohaghegh Mohammad Ali, Jaliani Hossein Zarei, Faridnia Roghiyeh, Kalani Hamed
Faculty of Para-Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ; Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Medical Parasitology and Mycology Department, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
J Parasit Dis. 2016 Dec;40(4):1571-1574. doi: 10.1007/s12639-015-0730-9. Epub 2016 Jan 14.
Cutaneous leishmaniasis is one of the important skin diseases with diverse clinical manifestations. With an incidence of 0.7-1.3 million cases annually, this disease is often reported from six countries, including Iran. Accordingly, the purpose of this study was to evaluate the anti-leishmanial effect of the three plant hydroalcoholic extracts including fleawort ( L.), savory ( L.) and tarragon ( L.) on promastigotes. The hydroalcoholic extract from each plant was extracted and its anti-leishmanial effect was evaluated in different concentrations (100-1000 µg/ml) and at various hours (24, 48 and 72 h). Savory herb inhibitory concentration 50 % (IC) at 24, 48 and 72 h was 790.81, 398.11 and 298.42 µg/ml, respectively. In addition, tarragon herb IC at 24, 48 and 72 h was 962.03, 688.36 and 585.51 µg/ml, respectively. Moreover, the fleawort extract was showed the lowest effect, considering that its effect at the concentration of 1000 µg/ml was 48 % after 72 h ( > 0.05). Furthermore, the statistical analysis showed a significant difference for interaction between concentration and time regarding the tarragon and savory extracts with a value of lower than 0.05. According to the results, the anti-leishmanial effect of the tarragon and savory extracts may make it possible to use them in the treatment of cutaneous leishmaniasis as a complementary or alternative therapy; however, further studies are necessary and should be evaluated in cell culture and in vivo conditions to confirm it.
皮肤利什曼病是一种临床表现多样的重要皮肤病。该病年发病率为70万至130万例，伊朗等六个国家常有病例报告。因此，本研究旨在评估三种植物水醇提取物，即泽漆（大戟属）、夏香薄荷（薄荷属）和龙蒿（蒿属）对前鞭毛体的抗利什曼作用。从每种植物中提取水醇提取物，并在不同浓度（100 - 1000微克/毫升）和不同时间（24、48和72小时）评估其抗利什曼作用。夏香薄荷在24、48和72小时的半数抑制浓度（IC50）分别为790.81、398.11和298.42微克/毫升。此外，龙蒿在24、48和72小时的IC50分别为962.03、688.36和585.51微克/毫升。此外，泽漆提取物的效果最低，因为其在1000微克/毫升浓度下72小时后的效果为48%（P>0.05）。此外，统计分析表明，龙蒿和夏香薄荷提取物的浓度和时间之间的相互作用存在显著差异，P值低于0.05。根据结果，龙蒿和夏香薄荷提取物的抗利什曼作用可能使其作为补充或替代疗法用于皮肤利什曼病的治疗成为可能；然而，需要进一步研究，并应在细胞培养和体内条件下进行评估以证实这一点。
