Department of Biotechnology, Era University, Lucknow, Uttar Pradesh, India.
Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
Free Radic Res. 2020 Sep;54(8-9):662-669. doi: 10.1080/10715762.2020.1825704.
There are few reports revealing association between iron intake and environmental lead exposure during pregnancy. Therefore, the present study was undertaken to investigate the effect of iron supplementation on biochemical modulation of certain lead toxicity markers associated with pregnancy. Iron and folic acid supplementations were given to 250 pregnant anemic women (mild = 100, moderate = 100 and severe = 50) and 100 age matched nonanemic pregnant women as controls for 100 days. Lead (Pb) toxicity markers, enzymatic and nonenzymatic antioxidant were estimated as per standard protocols. The levels of Pb, serum transferrin receptors (sTfR), zinc protoporphyrin (ZPP), δ-aminolevulinic acid (δ-ALA, both in blood and urine) were found significantly increased in all pretreated subjects and these were decreased after oral iron supplementation. Iron-deficient pregnant women reflected a significant increase in lipid peroxide levels (LPO) and protein carbonyl levels (PC) which were found to be further increased after iron supplementation. The levels of iron (Fe), haemoglobin (Hb), ferritin, delta aminolevulinic acid dehydratase (δ-ALAD), catalase (CAT), superoxide dismutase (SOD), and reduced glutathione levels (GSH) were significantly decreased in pretreated groups and these parameters were found significantly increased in all supplemented subjects after treatment. Antioxidant vitamins C and E were found significantly decreased in all post treated groups. Our observation suggests that recommended iron dose is not only effective for blood indices parameters, but it also decreases Pb concentrations in the blood during pregnancy. However, further studies with larger sample size are needed to confirm these findings.
关于铁摄入量与妊娠期间环境铅暴露之间的关联，鲜有报道。因此，本研究旨在调查铁补充对与妊娠相关的某些铅毒性标志物的生化调节的影响。给 250 名患有贫血的孕妇（轻度=100，中度=100，重度=50）和 100 名年龄匹配的非贫血孕妇补充铁和叶酸作为对照，共 100 天。按照标准方案评估铅（Pb）毒性标志物、酶和非酶抗氧化剂。所有预处理受试者的血液和尿液中的铅（Pb）、血清转铁蛋白受体（sTfR）、锌原卟啉（ZPP）、δ-氨基乙酰丙酸（δ-ALA）水平均显著升高，经口服铁补充后这些水平降低。缺铁的孕妇反映出脂质过氧化物（LPO）和蛋白质羰基（PC）水平显著增加，补充铁后这些水平进一步增加。铁（Fe）、血红蛋白（Hb）、铁蛋白、δ-氨基乙酰丙酸脱水酶（δ-ALAD）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）水平在预处理组中显著降低，所有补充组在治疗后这些参数均显著增加。抗氧化维生素 C 和 E 在所有治疗后组中均显著降低。我们的观察表明，推荐的铁剂量不仅对血液指标参数有效，而且还可以降低妊娠期间血液中的 Pb 浓度。然而，需要进行更大样本量的进一步研究来证实这些发现。
