Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences,·Key Laboratory of Animal Diseases, Diagnostics, and Immunology, Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infections Diseases and Zoonoses, Yangzhou, Jiangsu, China.
Virus Genes. 2020 Dec;56(6):781-784. doi: 10.1007/s11262-020-01796-8. Epub 2020 Sep 22.
Porcine circovirus-like agents comprise two types of viruses: porcine circovirus-like viruses (P1, P2, P3, and P4) and porcine circovirus-like mini agents (PCVL258, PCVL264, PCVL201, and PCVL347). Of these, P1 has been identified in pigs, cattle, goats, and rabbits in China; P2, P3, P4, PCVL258, and PCVL264 have been identified in pigs; and PCVL201 and PCVL347 have been identified in cattle. The purpose of this study was to determine whether dogs and cats have been exposed to porcine circovirus-like agents. We screened 158 serum samples from diseased dogs and 41 from cats in China by PCR and nucleotide sequencing. In dogs, approximately 18% (n = 28) were positive for P1, 17% (n = 26) for PCVL258, and 9% (n = 14) for PCVL264; in cats, 17.1% (n = 7) were positive for P1, 9.8% (n = 4) for P4, and 14.6% (n = 6) for PCVL258. The P1 genomes in this study consisted of 648 nucleotides (nt) and shared 96.8 to 100% nt identity with other P1 genomes in GenBank. The P4 genome shared 98.3 to 100% nt identity with other reported P4 genomes, and PCVL258 and PCVL264 showed 100% nt identity with previously reported genomes. To our knowledge, this is the first report on molecular characterization of porcine circovirus-like agents in dogs and cats. Further studies are needed to clarify the epidemiology, evolution, and pathogenesis of porcine circovirus-like agents in dogs and cats.
猪圆环病毒样病毒（P1、P2、P3 和 P4）和猪圆环病毒样微小病毒（PCVL258、PCVL264、PCVL201 和 PCVL347）。其中，在中国的猪、牛、山羊和兔中已鉴定出 P1；在猪中已鉴定出 P2、P3、P4、PCVL258 和 PCVL264；在牛中已鉴定出 PCVL201 和 PCVL347。本研究旨在确定犬和猫是否接触过猪圆环病毒样病毒。我们通过 PCR 和核苷酸测序筛查了中国 158 份患病犬血清样本和 41 份猫血清样本。在犬中，约 18%（n=28）的 P1 阳性，17%（n=26）的 PCVL258 阳性，9%（n=14）的 PCVL264 阳性；在猫中，17.1%（n=7）的 P1 阳性，9.8%（n=4）的 P4 阳性，14.6%（n=6）的 PCVL258 阳性。本研究中的 P1 基因组由 648 个核苷酸（nt）组成，与 GenBank 中其他 P1 基因组的 nt 同一性为 96.8%至 100%。P4 基因组与其他已报道的 P4 基因组的 nt 同一性为 98.3%至 100%，PCVL258 和 PCVL264 与之前报道的基因组完全相同。据我们所知，这是首次报道犬和猫中猪圆环病毒样病毒的分子特征。需要进一步的研究来阐明犬和猫中猪圆环病毒样病毒的流行病学、进化和发病机制。
