Muscle Research Unit, Experimental and Clinical Research Center, a joint cooperation of Charité, Universitätsmedizin Berlin and the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Stem Cell Res. 2020 Oct;48:101987. doi: 10.1016/j.scr.2020.101987. Epub 2020 Sep 8.
We describe the generation and characterization of three pairs of human induced pluripotent stem cell (hiPSC) lines reprogrammed from myoblasts and from peripheral blood mononuclear cells (PBMCs) of the same donor. All donors were free of neuromuscular disorders, female and between 47 and 50 years of age. For reprogramming we used Sendai-virus delivery of the four Yamanaka factors. The pluripotent identity of the hiPSC lines was confirmed by the expression of pluripotency markers and their capacity to differentiate into all three germ layers. These hiPSCs constitute a tool to study tissue of origin specific differences in the identity of hiPSCs.
我们描述了三对人类诱导多能干细胞（hiPSC）系的产生和特征，这些细胞系是由同一供体的成肌细胞和外周血单核细胞（PBMC）重新编程而来。所有供者均无神经肌肉疾病，均为女性，年龄在 47 至 50 岁之间。我们使用 Sendai 病毒传递了四个 Yamanaka 因子进行重编程。hiPSC 系的多能性通过多能性标志物的表达及其分化为三个胚层的能力得到确认。这些 hiPSC 构成了研究 hiPSC 起源组织特异性差异的工具。
