Metzler Eric, Telugu Narasimha, Diecke Sebastian, Spuler Simone, Escobar Helena
Muscle Research Unit, Experimental and Clinical Research Center, A Joint Cooperation of Charité, Universitätsmedizin Berlin and the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Stem Cell Res. 2020 Oct;48:101998. doi: 10.1016/j.scr.2020.101998. Epub 2020 Sep 17.
We describe the generation and characterization of two human induced pluripotent stem cell (hiPSCs) lines reprogrammed from myoblasts and from peripheral blood mononuclear cells (PBMCs) from the same donor. The donor was free of neuromuscular disorders, male and 18 years of age. For reprogramming we used Sendai-virus delivery of the four Yamanaka factors. The pluripotent identity of the hiPSC lines was confirmed by the expression of pluripotency markers and their capacity to differentiate into all three germ layers. These hiPSCs constitute a tool to study tissue of origin specific differences in the identity of hiPSCs.
我们描述了从同一位供体的成肌细胞和外周血单核细胞（PBMC）重编程而来的两个人类诱导多能干细胞（hiPSC）系的产生及特性。该供体无神经肌肉疾病，为18岁男性。我们使用仙台病毒递送四种山中因子进行重编程。通过多能性标志物的表达及其分化为所有三个胚层的能力，证实了hiPSC系的多能特性。这些hiPSC构成了一种工具，用于研究hiPSC身份中组织来源特异性差异。
