NMI Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany.
NMI Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany.
Stem Cell Res. 2021 Jul;54:102427. doi: 10.1016/j.scr.2021.102427. Epub 2021 Jun 11.
Peripheral-blood derived CD34 hematopoietic stem and progenitor cells were isolated from a 49-year old male donor and were successfully reprogrammed into human induced pluripotent stem cells (hiPSCs) using integration-free episomal vectors. The hiPSC line exhibited a typical stem cell-like morphology and endogenously expressed several pluripotency markers by concomitant loss of exogenous reprogramming vectors. Genomic integrity was confirmed by microarray-based comparative genomic hybridization (array CGH). Further analysis affirmed the ability of this hiPSC line to differentiate into all three germ layers. Thus, the reported cell line may serve as a healthy control for disease modeling.
从一位 49 岁男性供者的外周血中分离出 CD34 造血干/祖细胞，并用无整合的附加体载体成功将其重编程为人类诱导多能干细胞（hiPSC）。hiPSC 系表现出典型的干细胞样形态，并且通过同时丢失外源性重编程载体而内源性表达几种多能性标志物。通过基于微阵列的比较基因组杂交（array CGH）确认基因组完整性。进一步的分析证实了该 hiPSC 系分化为三个胚层的能力。因此，该报道的细胞系可用作疾病建模的健康对照。
