Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia.
Federal Scientific Research Center "Crystallography and Photonics", Russian Academy of Sciences, 119333 Moscow, Russia.
Molecules. 2020 Sep 19;25(18):4302. doi: 10.3390/molecules25184302.
Theranostic approach is currently among the fastest growing trends in cancer treatment. It implies the creation of multifunctional agents for simultaneous precise diagnosis and targeted impact on tumor cells. A new type of theranostic complexes was created based on NaYF: Yb,Tm upconversion nanoparticles coated with polyethylene glycol and functionalized with the HER2-specific recombinant targeted toxin DARPin-LoPE. The obtained agents bind to HER2-overexpressing human breast adenocarcinoma cells and demonstrate selective cytotoxicity against this type of cancer cells. Using fluorescent human breast adenocarcinoma xenograft models, the possibility of intravital visualization of the UCNP-based complexes biodistribution and accumulation in tumor was demonstrated.
治疗策略目前是癌症治疗中发展最快的趋势之一。它意味着创造多功能制剂，以实现对肿瘤细胞的精确诊断和靶向作用。基于上转换纳米粒子 NaYF: Yb,Tm 并包覆聚乙二醇和 HER2 特异性重组靶向毒素 DARPin-LoPE 进行功能化，合成了一种新型治疗策略复合物。所获得的制剂与过表达 HER2 的人乳腺腺癌细胞结合，并对这种类型的癌细胞表现出选择性细胞毒性。利用荧光人乳腺腺癌异种移植模型，证明了基于上转换纳米粒子的复合物在肿瘤中的生物分布和积累的活体可视化的可能性。
