Vojtkova Barbora, Spitzova Tatiana, Votypka Jan, Lestinova Tereza, Kominkova Iveta, Hajkova Michaela, Santos-Mateus David, Miles Michael A, Volf Petr, Sadlova Jovana
Department of Parasitology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
Department of Cell Biology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
Microorganisms. 2020 Sep 20;8(9):1440. doi: 10.3390/microorganisms8091440.
The clinical manifestation of leishmaniases depends on parasite species, host genetic background, and immune response. Manifestations of human leishmaniases are highly variable, ranging from self-healing skin lesions to fatal visceral disease. The scope of standard model hosts is insufficient to mimic well the wide disease spectrum, which compels the introduction of new model animals for leishmaniasis research. In this article, we study the susceptibility of three Asian rodent species ( and ) to and The external manifestation of the disease, distribution, as well as load of parasites and infectiousness to natural sand fly vectors, were compared with standard models, BALB/c mice and . No significant differences were found in disease outcomes in animals inoculated with sand fly- or culture-derived parasites. All Asian rodent species were highly susceptible to . showed the non-healing phenotype with the progressive growth of ulcerative lesions and massive parasite loads. and represented the healing phenotype, the latter with high infectiousness to vectors, mimicking best the character of natural reservoir hosts. Both, and were also highly susceptible to . having wider parasite distribution and higher parasite loads and infectiousness than standard model animals.
利什曼病的临床表现取决于寄生虫种类、宿主遗传背景和免疫反应。人类利什曼病的表现高度可变，从自愈性皮肤病变到致命的内脏疾病。标准模型宿主的范围不足以很好地模拟广泛的疾病谱，这促使引入新的利什曼病研究模型动物。在本文中，我们研究了三种亚洲啮齿动物物种（和）对和的易感性。将疾病的外在表现、分布以及寄生虫负荷和对天然白蛉媒介的传染性与标准模型BALB/c小鼠和进行了比较。在用白蛉或培养衍生的寄生虫接种的动物中，疾病结果未发现显著差异。所有亚洲啮齿动物物种对均高度易感。表现出不愈合的表型，溃疡性病变逐渐生长且寄生虫负荷量大。和表现出愈合表型，后者对媒介具有高传染性，最能模拟天然储存宿主的特征。和对也高度易感。其寄生虫分布比标准模型动物更广，寄生虫负荷和传染性更高。
