Shi Kaiyun, Yang Guang, He Liang, Yang Bo, Li Qin, Yi Sha
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.
The Key Laboratory of Biochemical Utilization of Zhejiang Province, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou, China.
J Food Biochem. 2020 Nov;44(11):e13482. doi: 10.1111/jfbc.13482. Epub 2020 Sep 22.
Two new water-soluble polysaccharide fractions (SCP II-1 and SCP II-2) were obtained from silkworm cordycepsusing DEAE Sepharose FF and Superose 6 columns chromatography. The characterization of their basic structure was studied by high performance liquid chromatography, high performance ion chromatography, infrared spectroscopy and atomic force microscope (AFM). The results showed that the molecular weight of SCP II-1 and SCP II-2 were 35.2 kDa and 23.4 kDa, and they were mainly composed of ribose, mannose, glucose, and galactose in molar ratio of 1.0:27.38:8.52:17.99 and 1.0:21.21:1.95:14.28, respectively. The AFM topography confirmed the highly branched chain conformation of SCP II-1, while SCP II-2 had more polymerized chain morphology. These two fractions possessed excellent antioxidant and antitumor activities, especially SCP II-1 showed better inhibition than SCP II-2. Those data suggested that purified polysaccharides from silkworm cordyceps have potential application in functional food and pharmaceutical industry. PRACTICAL APPLICATIONS: Silkworm cordyceps is cultivated on the 5th instar larvae inoculated with Cordyceps sp. and the functions of cordyceps have been reported recently. The separation and purification of silkworm cordyceps polysaccharide is helpful to better exert important biological functions. The study on the structure-function relations of polysaccharides will be useful to the application of polysaccharides in functional food and pharmaceutical industry.
利用DEAE Sepharose FF和Superose 6柱色谱法从蚕虫草中获得了两种新的水溶性多糖组分（SCP II-1和SCP II-2）。通过高效液相色谱、高效离子色谱、红外光谱和原子力显微镜（AFM）对其基本结构进行了表征。结果表明，SCP II-1和SCP II-2的分子量分别为35.2 kDa和23.4 kDa，它们主要由核糖、甘露糖、葡萄糖和半乳糖组成，摩尔比分别为1.0:27.38:8.52:17.99和1.0:21.21:1.95:14.28。AFM形貌证实了SCP II-1具有高度分支的链构象，而SCP II-2具有更多的聚合链形态。这两个组分具有优异的抗氧化和抗肿瘤活性，尤其是SCP II-1的抑制作用比SCP II-2更好。这些数据表明，从蚕虫草中纯化的多糖在功能性食品和制药工业中具有潜在的应用价值。实际应用：蚕虫草是在接种了虫草菌的5龄幼虫上培育的，虫草的功能最近已有报道。蚕虫草多糖的分离纯化有助于更好地发挥其重要的生物学功能。对多糖结构-功能关系的研究将有助于多糖在功能性食品和制药工业中的应用。
