School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 518055, China.
Skirball Institute, Department of Neuroscience and Physiology, Department of Anesthesiology, New York University School of Medicine, New York, NY, 10016, USA.
Nat Commun. 2020 Sep 23;11(1):4819. doi: 10.1038/s41467-020-18592-5.
In many parts of the nervous system, experience-dependent refinement of neuronal circuits predominantly involves synapse elimination. The role of sleep in this process remains unknown. We investigated the role of sleep in experience-dependent dendritic spine elimination of layer 5 pyramidal neurons in the visual (V1) and frontal association cortex (FrA) of 1-month-old mice. We found that monocular deprivation (MD) or auditory-cued fear conditioning (FC) caused rapid spine elimination in V1 or FrA, respectively. MD- or FC-induced spine elimination was significantly reduced after total sleep or REM sleep deprivation. Total sleep or REM sleep deprivation also prevented MD- and FC-induced reduction of neuronal activity in response to visual or conditioned auditory stimuli. Furthermore, dendritic calcium spikes increased substantially during REM sleep, and the blockade of these calcium spikes prevented MD- and FC-induced spine elimination. These findings reveal an important role of REM sleep in experience-dependent synapse elimination and neuronal activity reduction.
在神经系统的许多部位，神经元回路的经验依赖性精细化主要涉及突触消除。睡眠在这个过程中的作用尚不清楚。我们研究了睡眠在 1 个月大的小鼠的视觉（V1）和额前联合皮层（FrA）的第 5 层锥体神经元的经验依赖性树突棘消除中的作用。我们发现，单眼剥夺（MD）或听觉提示的恐惧条件反射（FC）分别导致 V1 或 FrA 中的快速棘突消除。完全睡眠剥夺或 REM 睡眠剥夺后，MD 或 FC 诱导的棘突消除明显减少。完全睡眠剥夺或 REM 睡眠剥夺也阻止了 MD 和 FC 诱导的对视觉或条件听觉刺激的神经元活动减少。此外，在 REM 睡眠期间树突钙峰显著增加，并且这些钙峰的阻断阻止了 MD 和 FC 诱导的棘突消除。这些发现揭示了 REM 睡眠在经验依赖性突触消除和神经元活动减少中的重要作用。
