Pranaw Kumar, Pidlisnyuk Valentina, Trögl Josef, Malinská Hana
Department of Environmental Chemistry and Technology, Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic.
Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Biology (Basel). 2020 Sep 22;9(9):305. doi: 10.3390/biology9090305.
Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop × () in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caused by salinity, pH, temperature, and lead (Pb). Selected strains were further assessed and screened for plant growth-promoting attributes. The isolate showing the most potential, KP-14, was tested for enhancement of growth in contaminated soil under greenhouse conditions. It was found to be highly tolerant to diverse abiotic stresses, exhibiting tolerance to salinity (0-15%), pH (4-8), temperature (4-50 °C), and Pb (up to 1200 ppm). The association of KP-14 with resulted in a significant ( ≤ 0.001) impact on biomass enhancement: the total shoot and dry root weights were significantly enhanced by 77.7% and 55.5%, respectively. The significant enhancement of biomass parameters by application of KP-14 strongly supports the use of this strain as a biofertilizer for the improvement of plant growth in metal-contaminated soils.
利用植物促生细菌（PGPB）在军事和采矿后的场地种植生物燃料作物 × （），是对受金属污染土壤进行生物修复的一种有前景的方法。在本研究中，从受污染土壤中分离出PGPB，并筛选其对盐度、pH值、温度和铅（Pb）引起的非生物胁迫的耐受性。对筛选出的菌株进一步评估并筛选其促进植物生长的特性。对表现出最大潜力的分离株KP-14在温室条件下进行了在受污染土壤中促进 生长的测试。发现它对多种非生物胁迫具有高度耐受性，能耐受盐度（0-15%）、pH值（4-8）、温度（4-50°C）和铅（高达1200 ppm）。KP-14与 的关联对生物量增加产生了显著影响（≤0.001）：地上部总重量和干根重量分别显著增加了77.7%和55.5%。应用KP-14显著提高了 生物量参数，有力地支持了将该菌株用作生物肥料以改善金属污染土壤中植物生长的用途。
