Zuccarello M, Sawaya R, Ray M B
Cancer. 1987 Aug 15;60(4):804-9. doi: 10.1002/1097-0142(19870815)60:4<804::aid-cncr2820600416>3.0.co;2-u.
Using light microscopy and immunoperoxidase methods (PAP), the presence of alpha-1-proteinase inhibitor (API) was studied in seventeen brain tumors and four normal brain samples. The brain tumors included four glioblastomas, five low-grade gliomas, two metastatic lung carcinomas, two acoustic schwannomas, and four meningiomas. Normal brain displayed a finely granular intracytoplasmic staining confined to neuronal cells. Glial cells were negative for API. Fifteen of the 17 brain tumors were positive for API. Only two of five low-grade gliomas were negative for API. Glioblastoma and metastatic tumors exhibited the strongest positivity followed by acoustic neuroma, meningioma, and low-grade glioma. All positive samples exhibited finely granular intracytoplasmic API, and 50% exhibited extracellular API positivity. Metastatic and glioblastoma tumors demonstrated prominent extracellular API staining. Our results support the concept of a local production of API by brain tumors.
采用光学显微镜和免疫过氧化物酶法（PAP），对17例脑肿瘤和4例正常脑样本中α1 -蛋白酶抑制剂（API）的存在情况进行了研究。脑肿瘤包括4例胶质母细胞瘤、5例低级别胶质瘤、2例肺转移癌、2例听神经瘤和4例脑膜瘤。正常脑显示神经元细胞内有细颗粒状胞质染色。胶质细胞对API呈阴性。17例脑肿瘤中有15例API呈阳性。5例低级别胶质瘤中只有2例API呈阴性。胶质母细胞瘤和转移瘤的阳性最强，其次是听神经瘤、脑膜瘤和低级别胶质瘤。所有阳性样本均显示细颗粒状胞质内API，50%显示细胞外API阳性。转移瘤和胶质母细胞瘤显示出明显的细胞外API染色。我们的结果支持脑肿瘤局部产生API的概念。
