Mosser D D, Van Oostrom J, Bols N C
J Cell Physiol. 1987 Jul;132(1):155-60. doi: 10.1002/jcp.1041320122.
Thermotolerance was studied in the rainbow trout fibroblast cell line RTG-2. RTG-2 cultures that had been incubated at 28 degrees C for 24 h were better able to withstand ultimately lethal temperatures above 28 degrees C than RTG-2 cultures that had been maintained at the routine growth temperature of 22 degrees C. This thermotolerance developed rapidly between 3 and 6 h and was fully developed by 24 h at 28 degrees C. After development for 24 hr at 28 degrees C, thermotolerance showed little change over 72 h at 0 and 5 degrees C but approximately a 40 and 60% reduction at 10 and 22 degrees C, respectively. This is the first demonstration of heat-induced thermal resistance in the cells of a poikilothermic vertebrate.
在虹鳟鱼成纤维细胞系RTG-2中研究了耐热性。与在常规生长温度22℃下培养的RTG-2培养物相比，在28℃下孵育24小时的RTG-2培养物更能承受高于28℃的最终致死温度。这种耐热性在3至6小时之间迅速发展，并在28℃下24小时时完全形成。在28℃下培养24小时后，耐热性在0℃和5℃下72小时内变化不大，但在10℃和22℃下分别降低约40%和60%。这是首次在变温脊椎动物的细胞中证明热诱导的热抗性。
