Mosser D D, Bols N C
Department of Biology, University of Waterloo, Ontario, Canada.
J Comp Physiol B. 1988;158(4):457-67. doi: 10.1007/BF00691143.
The role of heat-shock protein synthesis in the development of thermotolerance by rainbow trout fibroblasts was examined. During the first 6 h after being shifted from 22 degrees C to 28 degrees C, cells of the rainbow trout fibroblast line, RTG-2, rapidly synthesized the major heat-shock proteins (hsps), hsps 87, 70 and 27, and developed tolerance to 32 degrees C. After 24 h at 28 degrees C hsp synthesis was drastically reduced but thermotolerance was maintained. If these thermotolerant cells were shifted to 32 degrees C, hsp synthesis continued at a very low level, but if they were subsequently returned to 22 degrees C, synthesis of hsps 70 and 27 was induced again. The addition of actinomycin D during the first 6 h at 28 degrees C prevented hsp synthesis and the development of thermotolerance. The presence of actinomycin D during the incubation of thermotolerant cultures at 32 degrees C blocked the reinitiation of hsps synthesis at 22 degrees C but had no effect on survival. Therefore, the hsps that accumulated at 28 degrees C were sufficient to allow cells to survive a subsequent thermal stress at 32 degrees C.
研究了热休克蛋白合成在虹鳟鱼成纤维细胞耐热性形成中的作用。在从22℃转移到28℃后的最初6小时内，虹鳟鱼成纤维细胞系RTG-2的细胞迅速合成主要的热休克蛋白（hsps），即hsps 87、70和27，并产生了对32℃的耐受性。在28℃培养24小时后，hsp合成急剧减少，但耐热性得以维持。如果将这些耐热细胞转移到32℃，hsp合成会在非常低的水平上继续，但如果随后将它们再转移回22℃，hsps 70和27的合成会再次被诱导。在28℃的最初6小时内添加放线菌素D可阻止hsp合成和耐热性的形成。在32℃培养耐热培养物期间存在放线菌素D会阻断在22℃时hsps合成的重新启动，但对细胞存活没有影响。因此，在28℃积累的hsps足以使细胞在随后32℃的热应激中存活。
