Dise T, Brunell P A
J Clin Microbiol. 1987 Jun;25(6):987-90. doi: 10.1128/jcm.25.6.987-990.1987.
Much nonspecific activity in enzyme immunoassay is due to reactions between anti-bovine antibodies which are commonly present in human serum samples and residual calf serum in viral antigens. Calf serum is used in cultured cells which are used to produce viral antigens. Addition of bovine proteins to serum samples to be tested for viral antibodies reduces this activity.
酶免疫测定中许多非特异性活性是由于人血清样本中常见的抗牛抗体与病毒抗原中残留的小牛血清之间的反应所致。小牛血清用于培养用于生产病毒抗原的细胞。向要检测病毒抗体的血清样本中添加牛蛋白可降低这种活性。