Salonen J, Vainionpää R, Halonen P
Arch Virol. 1986;91(1-2):93-106. doi: 10.1007/BF01316731.
Purified measles virions, nucleocapsid protein and crude lysate of measles infected cells were labelled with horseradish peroxidase, and used for the detection of IgM and IgG antibodies to measles virus by direct enzyme immunoassay. The assays consisted of three layers: anti-human IgM or IgG immunoglobulins on solid-phase, test serum specimen and enzyme labelled viral antigen. For the expression of the results, a standard curve was included in each test and the O.D. values were changed to arbitrary antibody units. Specificity and sensitivity of the assays were compared with indirect EIAs. The specificity studies included a collection of serum specimens containing either rheumatoid factor, antinuclear antibodies or IgM antibody specific for other viruses. The assays proved both reliable and simple to perform and sensitivity was slightly higher than that for indirect EIAs. However, specificity was dependent on the purity of the viral antigens. When crude infected cell lysate antigen was used, some nonspecific results were obtained, particularly with serum specimens containing antinuclear antibodies. When virion or nucleocapsid protein were used, no nonspecific reactions were obtained.
将纯化的麻疹病毒粒子、核衣壳蛋白以及麻疹感染细胞的粗裂解物用辣根过氧化物酶进行标记，然后通过直接酶免疫测定法用于检测针对麻疹病毒的IgM和IgG抗体。这些测定法包含三层：固相上的抗人IgM或IgG免疫球蛋白、测试血清标本以及酶标记的病毒抗原。为了表达结果，每次测试都包含一条标准曲线，并且光密度值被换算为任意抗体单位。将这些测定法的特异性和敏感性与间接酶免疫测定法进行比较。特异性研究包括收集含有类风湿因子、抗核抗体或针对其他病毒的IgM抗体的血清标本。这些测定法被证明既可靠又易于操作，并且敏感性略高于间接酶免疫测定法。然而，特异性取决于病毒抗原的纯度。当使用粗感染细胞裂解物抗原时，会得到一些非特异性结果，尤其是使用含有抗核抗体的血清标本时。当使用病毒粒子或核衣壳蛋白时，则未获得非特异性反应。
