烟曲霉原生质体和亚细胞组分中的几丁质生物合成
Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus.
作者信息
Archer D B
出版信息
Biochem J. 1977 Jun 15;164(3):653-8. doi: 10.1042/bj1640653.
Abstract
The biosynthesis of chitin has been obtained in broken mycelia and protoplasts of the fungus Aspergillus fumigatus. The specific activity of chitin synthase (EC 2.4.1.16) in a membrane preparation from protoplasts derived from the hyphal tips of A. fumigatus was 26.8-fold greater than that of the chitin synthase in broken mycelia, indicating that the active chitin synthase is located primarily in a membrane-bound site at the hyphal tip. Polyoxin D was a potent competitive inhibitor of the enzyme, having Ki 5.2 +/- 0.8 micron with respect to the natural substrate UDP-N-acetyl-D-glucosamine, which has Km 1.58 mM.
摘要
在烟曲霉的破碎菌丝体和原生质体中已实现几丁质的生物合成。来自烟曲霉菌丝尖端的原生质体制备的膜制剂中几丁质合酶（EC 2.4.1.16）的比活性比破碎菌丝体中的几丁质合酶高26.8倍，这表明活性几丁质合酶主要位于菌丝尖端的膜结合部位。多氧霉素D是该酶的有效竞争性抑制剂，相对于天然底物UDP-N-乙酰-D-葡糖胺（Km为1.58 mM），其Ki为5.2±0.8微米。
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